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. 1997 Sep;105(Suppl 5):1285–1289. doi: 10.1289/ehp.97105s51285

Free radical activity of PM10: iron-mediated generation of hydroxyl radicals.

K Donaldson 1, D M Brown 1, C Mitchell 1, M Dineva 1, P H Beswick 1, P Gilmour 1, W MacNee 1
PMCID: PMC1470141  PMID: 9400739

Abstract

The purpose of this study was to test the hypothesis that particulate matter < or = 10 microns in aerodynamic diameter (PM10) particles have the ability to generate free radical activity at their surface. We collected PM10 filters from the Edinburgh, United Kingdom, Enhanced Urban Network sampling site, removed particles from the filter, and tested their ability to cause free radical damage to supercoiled plasmid DNA. We found that the PM10 particles did cause damage to the DNA that was mediated by hydroxyl radicals, as shown by inhibition of the injury with mannitol. The PM10-associated hydroxyl radical activity was confirmed using a high-performance liquid chromatography-based assay to measure the hydroxyl radical adduct of salicylic acid. Desferrioxamine abolished the hydroxyl radical-mediated injury, which suggests that iron was involved. Analysis of PM10 filters confirmed the presence of large amounts of iron and leaching studies confirmed that the PM10 samples could release substantial amounts of Fe(III) and lesser amounts of Fe(II). To investigate the size of the particles involved in the hydroxyl radical injury, we centrifuged the suspension of PM10 to clarity, tested the clear supernatant, and found that it had all of the suspension activity. We conclude, therefore, that the free radical activity is derived either from a fraction that is not centrifugeable on a bench centrifuge, or that the radical generating system is released into solution.

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Selected References

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  1. Dalal N. S., Suryan M. M., Vallyathan V., Green F. H., Jafari B., Wheeler R. Detection of reactive free radicals in fresh coal mine dust and their implication for pulmonary injury. Ann Occup Hyg. 1989;33(1):79–84. doi: 10.1093/annhyg/33.1.79. [DOI] [PubMed] [Google Scholar]
  2. Dröge W., Schulze-Osthoff K., Mihm S., Galter D., Schenk H., Eck H. P., Roth S., Gmünder H. Functions of glutathione and glutathione disulfide in immunology and immunopathology. FASEB J. 1994 Nov;8(14):1131–1138. [PubMed] [Google Scholar]
  3. Dusseldorp A., Kruize H., Brunekreef B., Hofschreuder P., de Meer G., van Oudvorst A. B. Associations of PM10 and airborne iron with respiratory health of adults living near a steel factory. Am J Respir Crit Care Med. 1995 Dec;152(6 Pt 1):1932–1939. doi: 10.1164/ajrccm.152.6.8520758. [DOI] [PubMed] [Google Scholar]
  4. Ferin J., Oberdörster G., Penney D. P. Pulmonary retention of ultrafine and fine particles in rats. Am J Respir Cell Mol Biol. 1992 May;6(5):535–542. doi: 10.1165/ajrcmb/6.5.535. [DOI] [PubMed] [Google Scholar]
  5. Fubini B., Mollo L., Giamello E. Free radical generation at the solid/liquid interface in iron containing minerals. Free Radic Res. 1995 Dec;23(6):593–614. doi: 10.3109/10715769509065280. [DOI] [PubMed] [Google Scholar]
  6. Ghio A. J., Kennedy T. P., Stonehuerner J. G., Crumbliss A. L., Hoidal J. R. DNA strand breaks following in vitro exposure to asbestos increase with surface-complexed [Fe3+]. Arch Biochem Biophys. 1994 May 15;311(1):13–18. doi: 10.1006/abbi.1994.1202. [DOI] [PubMed] [Google Scholar]
  7. Gilmour P. S., Beswick P. H., Brown D. M., Donaldson K. Detection of surface free radical activity of respirable industrial fibres using supercoiled phi X174 RF1 plasmid DNA. Carcinogenesis. 1995 Dec;16(12):2973–2979. doi: 10.1093/carcin/16.12.2973. [DOI] [PubMed] [Google Scholar]
  8. Janssen Y. M., Heintz N. H., Mossman B. T. Induction of c-fos and c-jun proto-oncogene expression by asbestos is ameliorated by N-acetyl-L-cysteine in mesothelial cells. Cancer Res. 1995 May 15;55(10):2085–2089. [PubMed] [Google Scholar]
  9. Kennedy T. P., Dodson R., Rao N. V., Ky H., Hopkins C., Baser M., Tolley E., Hoidal J. R. Dusts causing pneumoconiosis generate .OH and produce hemolysis by acting as Fenton catalysts. Arch Biochem Biophys. 1989 Feb 15;269(1):359–364. doi: 10.1016/0003-9861(89)90118-5. [DOI] [PubMed] [Google Scholar]
  10. Li X. Y., Donaldson K., Brown D., MacNee W. The role of tumor necrosis factor in increased airspace epithelial permeability in acute lung inflammation. Am J Respir Cell Mol Biol. 1995 Aug;13(2):185–195. doi: 10.1165/ajrcmb.13.2.7626286. [DOI] [PubMed] [Google Scholar]
  11. Li X. Y., Donaldson K., Rahman I., MacNee W. An investigation of the role of glutathione in increased epithelial permeability induced by cigarette smoke in vivo and in vitro. Am J Respir Crit Care Med. 1994 Jun;149(6):1518–1525. doi: 10.1164/ajrccm.149.6.8004308. [DOI] [PubMed] [Google Scholar]
  12. Lund L. G., Aust A. E. Iron-catalyzed reactions may be responsible for the biochemical and biological effects of asbestos. Biofactors. 1991 Jun;3(2):83–89. [PubMed] [Google Scholar]
  13. Lund L. G., Williams M. G., Dodson R. F., Aust A. E. Iron associated with asbestos bodies is responsible for the formation of single strand breaks in phi X174 RFI DNA. Occup Environ Med. 1994 Mar;51(3):200–204. doi: 10.1136/oem.51.3.200. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Nyberg K., Johansson U., Johansson A., Camner P. Phagolysosomal pH in alveolar macrophages. Environ Health Perspect. 1992 Jul;97:149–152. doi: 10.1289/ehp.9297149. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Oberdorster G., Gelein R. M., Ferin J., Weiss B. Association of particulate air pollution and acute mortality: involvement of ultrafine particles? Inhal Toxicol. 1995 Jan-Feb;7(1):111–124. doi: 10.3109/08958379509014275. [DOI] [PubMed] [Google Scholar]
  16. Pope C. A., 3rd, Bates D. V., Raizenne M. E. Health effects of particulate air pollution: time for reassessment? Environ Health Perspect. 1995 May;103(5):472–480. doi: 10.1289/ehp.95103472. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rahman I., Morrison D., Donaldson K., MacNee W. Systemic oxidative stress in asthma, COPD, and smokers. Am J Respir Crit Care Med. 1996 Oct;154(4 Pt 1):1055–1060. doi: 10.1164/ajrccm.154.4.8887607. [DOI] [PubMed] [Google Scholar]
  18. Schwartz J. What are people dying of on high air pollution days? Environ Res. 1994 Jan;64(1):26–35. doi: 10.1006/enrs.1994.1004. [DOI] [PubMed] [Google Scholar]
  19. Seaton A., MacNee W., Donaldson K., Godden D. Particulate air pollution and acute health effects. Lancet. 1995 Jan 21;345(8943):176–178. doi: 10.1016/s0140-6736(95)90173-6. [DOI] [PubMed] [Google Scholar]
  20. Simeonova P. P., Luster M. I. Iron and reactive oxygen species in the asbestos-induced tumor necrosis factor-alpha response from alveolar macrophages. Am J Respir Cell Mol Biol. 1995 Jun;12(6):676–683. doi: 10.1165/ajrcmb.12.6.7539275. [DOI] [PubMed] [Google Scholar]
  21. Vallyathan V., Shi X. L., Dalal N. S., Irr W., Castranova V. Generation of free radicals from freshly fractured silica dust. Potential role in acute silica-induced lung injury. Am Rev Respir Dis. 1988 Nov;138(5):1213–1219. doi: 10.1164/ajrccm/138.5.1213. [DOI] [PubMed] [Google Scholar]

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