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. 1997 Nov 15;25(22):4639–4643. doi: 10.1093/nar/25.22.4639

Increased DNA binding and sequence discrimination of PNA oligomers containing 2,6-diaminopurine.

G Haaima 1, H F Hansen 1, L Christensen 1, O Dahl 1, P E Nielsen 1
PMCID: PMC147079  PMID: 9358176

Abstract

The synthesis of a diaminopurine PNA monomer, N-[N6-(benzyloxycarbonyl)-2,6-diaminopurine-9-yl] acetyl-N-(2-t-butyloxycarbonylaminoethyl)glycine, and the incorporation of this monomer into PNA oligomers are described. Substitution of adenine by diaminopurine in PNA oligomers increased the T m of duplexes formed with complementary DNA, RNA or PNA by 2.5-6.5 degrees C per diaminopurine. Furthermore, discrimination against mismatches facing the diaminopurine in the hybridizing oligomer is improved. Finally, a homopurine decamer PNA containing six diaminopurines is shown to form a (gel shift) stable strand displacement complex with a target in a 246 bp double-stranded DNA fragment.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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