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. 1997 Nov 15;25(22):4447–4454. doi: 10.1093/nar/25.22.4447

Oligonucleotide dendrimers: synthesis and use as polylabelled DNA probes.

M S Shchepinov 1, I A Udalova 1, A J Bridgman 1, E M Southern 1
PMCID: PMC147080  PMID: 9358151

Abstract

Oligonucleotide dendrimers were synthesized using a novel phosphoramidite synthon, tris-2,2,2-[3-(4,4'-dimethoxytrityloxy) propyloxymethyl]ethyl- N , N -diisopropylaminocyanethoxy phosphoramidite. Label, incorporated using [gamma-32P]ATP and polynucleotide kinase, was increased in proportion to the number of 5'-ends. There was a similar increase in signal when these multiply labelled oligonucleotides were used as probes to oligonucleotide arrays. A dendrimeric oligonucleotide was used successfully as a primer in the PCR. The strand bearing the dendrimer was resistant to degradation by T7 Gene 6 exonuclease making it easy to convert the double-stranded product of the PCR to a multiply-labelled, single-stranded probe.

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Selected References

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