Skip to main content
PMC home page
Genetics logoLink to Genetics
. 2004 May;167(1):343–355. doi: 10.1534/genetics.167.1.343

A physically anchored genetic map and linkage to avirulence reveals recombination suppression over the proximal region of Hessian fly chromosome A2.

Susanta K Behura 1, Fernando H Valicente 1, S Dean Rider Jr 1, Ming Shun-Chen 1, Scott Jackson 1, Jeffrey J Stuart 1
PMCID: PMC1470865  PMID: 15166159

Abstract

Resistance in wheat (Triticum aestivum) to the Hessian fly (Mayetiola destructor), a major insect pest of wheat, is based on a gene-for-gene interaction. Close linkage (3 +/- 2 cM) was discovered between Hessian fly avirulence genes vH3 and vH5. Bulked segregant analysis revealed two DNA markers (28-178 and 23-201) within 10 cM of these loci and only 3 +/- 2 cM apart. However, 28-178 was located in the middle of the short arm of Hessian fly chromosome A2 whereas 23-201 was located in the middle of the long arm of chromosome A2, suggesting the presence of severe recombination suppression over its proximal region. To further test that possibility, an AFLP-based genetic map of the Hessian fly genome was constructed. Fluorescence in situ hybridization of 20 markers on the genetic map to the polytene chromosomes of the Hessian fly indicated good correspondence between the linkage groups and the four Hessian fly chromosomes. The physically anchored genetic map is the first of any gall midge species. The proximal region of mitotic chromosome A2 makes up 30% of its length but corresponded to <3% of the chromosome A2 genetic map.

Full Text

The Full Text of this article is available as a PDF (282.4 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Altschul S. F., Madden T. L., Schäffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 1997 Sep 1;25(17):3389–3402. doi: 10.1093/nar/25.17.3389. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bantock C. R. Experiments on chromosome elimination in the gall midge, Mayetiola destructor. J Embryol Exp Morphol. 1970 Sep;24(2):257–286. [PubMed] [Google Scholar]
  3. Behura S. K., Nair S., Mohan M. Polymorphisms flanking the mariner integration sites in the rice gall midge (Orseolia oryzae Wood-Mason) genome are biotype-specific. Genome. 2001 Dec;44(6):947–954. doi: 10.1139/g01-090. [DOI] [PubMed] [Google Scholar]
  4. Boyko Elena, Kalendar Ruslan, Korzun Victor, Fellers John, Korol Abraham, Schulman Alan H., Gill Bikram S. A high-density cytogenetic map of the Aegilops tauschii genome incorporating retrotransposons and defense-related genes: insights into cereal chromosome structure and function. Plant Mol Biol. 2002 Mar-Apr;48(5-6):767–790. doi: 10.1023/a:1014831511810. [DOI] [PubMed] [Google Scholar]
  5. Denell R. E., Keppy D. O. The nature of genetic recombination near the third chromosome centromere or Drosophila melanogaster. Genetics. 1979 Sep;93(1):117–130. doi: 10.1093/genetics/93.1.117. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. El Bouhssini M., Hatchett J. H., Cox T. S., Wilde G. E. Genotypic interaction between resistance genes in wheat and virulence genes in the Hessian fly Mayetiola destructor (Diptera: Cecidomyiidae). Bull Entomol Res. 2001 Oct;91(5):327–331. doi: 10.1079/ber2001115. [DOI] [PubMed] [Google Scholar]
  7. Green M. M. Conversion as a possible mechanism of high coincidence values in the centromere region of Drosophila. Mol Gen Genet. 1975 Aug 5;139(1):57–66. doi: 10.1007/BF00267995. [DOI] [PubMed] [Google Scholar]
  8. Lander E. S., Green P., Abrahamson J., Barlow A., Daly M. J., Lincoln S. E., Newberg L. A., Newburg L. MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics. 1987 Oct;1(2):174–181. doi: 10.1016/0888-7543(87)90010-3. [DOI] [PubMed] [Google Scholar]
  9. Leach J. E., White F. F. Bacterial avirulence genes. Annu Rev Phytopathol. 1996;34:153–179. doi: 10.1146/annurev.phyto.34.1.153. [DOI] [PubMed] [Google Scholar]
  10. Michelmore R. W., Paran I., Kesseli R. V. Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci U S A. 1991 Nov 1;88(21):9828–9832. doi: 10.1073/pnas.88.21.9828. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Naber N., El Bouhssini M., Labhilili M., Udupa S. M., Nachit M. M., Baum M., Lhaloui S., Benslimane A., El Abbouyi H. Genetic variation among populations of the Hessian fly Mayetiola destructor (Diptera: Cecidomyiidae) in Morocco and Syria. Bull Entomol Res. 2000 Jun;90(3):245–252. doi: 10.1017/s0007485300000365. [DOI] [PubMed] [Google Scholar]
  12. Nicolas A. Relationship between transcription and initiation of meiotic recombination: toward chromatin accessibility. Proc Natl Acad Sci U S A. 1998 Jan 6;95(1):87–89. doi: 10.1073/pnas.95.1.87. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. ROBERTS P. A. DIFFERENCE IN THE BEHAVIOR OF EU- AND HETERO-CHROMATIN: CROSSING-OVER. Nature. 1965 Feb 13;205:725–726. doi: 10.1038/205725b0. [DOI] [PubMed] [Google Scholar]
  14. Rider Stanley Dean, Jr, Sun Weilin, Ratcliffe Roger H., Stuart Jeffrey J. Chromosome landing near avirulence gene vH13 in the Hessian fly. Genome. 2002 Oct;45(5):812–822. doi: 10.1139/g02-047. [DOI] [PubMed] [Google Scholar]
  15. Rossi M., Goggin F. L., Milligan S. B., Kaloshian I., Ullman D. E., Williamson V. M. The nematode resistance gene Mi of tomato confers resistance against the potato aphid. Proc Natl Acad Sci U S A. 1998 Aug 18;95(17):9750–9754. doi: 10.1073/pnas.95.17.9750. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Schulte S. J., Rider S. D., Jr, Hatchett J. H., Stuart J. J. Molecular genetic mapping of three X-linked avirulence genes, vH6, vH9 and vH13, in the Hessian fly. Genome. 1999 Oct;42(5):821–828. doi: 10.1139/g98-162. [DOI] [PubMed] [Google Scholar]
  17. Sinclair D. A. Crossing over between closely linked markers spanning the centromere of chromosome 3 in drosophila melanogaster. Genet Res. 1975 Oct;26(2):173–185. doi: 10.1017/s0016672300015974. [DOI] [PubMed] [Google Scholar]
  18. Stuart J. J., Schulte S. J., Hall P. S., Mayer K. M. Genetic mapping of Hessian fly avirulence gene vH6 using bulked segregant analysis. Genome. 1998 Oct;41(5):702–708. [PubMed] [Google Scholar]
  19. Sybenga J. Recombination and chiasmata: few but intriguing discrepancies. Genome. 1996 Jun;39(3):473–484. doi: 10.1139/g96-061. [DOI] [PubMed] [Google Scholar]
  20. Tanksley S. D., Ganal M. W., Prince J. P., de Vicente M. C., Bonierbale M. W., Broun P., Fulton T. M., Giovannoni J. J., Grandillo S., Martin G. B. High density molecular linkage maps of the tomato and potato genomes. Genetics. 1992 Dec;132(4):1141–1160. doi: 10.1093/genetics/132.4.1141. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Yu A., Zhao C., Fan Y., Jang W., Mungall A. J., Deloukas P., Olsen A., Doggett N. A., Ghebranious N., Broman K. W. Comparison of human genetic and sequence-based physical maps. Nature. 2001 Feb 15;409(6822):951–953. doi: 10.1038/35057185. [DOI] [PubMed] [Google Scholar]

Articles from Genetics are provided here courtesy of Oxford University Press

RESOURCES