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. 2004 Jun;167(2):797–813. doi: 10.1534/genetics.104.026658

The DrosDel collection: a set of P-element insertions for generating custom chromosomal aberrations in Drosophila melanogaster.

Edward Ryder 1, Fiona Blows 1, Michael Ashburner 1, Rosa Bautista-Llacer 1, Darin Coulson 1, Jenny Drummond 1, Jane Webster 1, David Gubb 1, Nicola Gunton 1, Glynnis Johnson 1, Cahir J O'Kane 1, David Huen 1, Punita Sharma 1, Zoltan Asztalos 1, Heiko Baisch 1, Janet Schulze 1, Maria Kube 1, Kathrin Kittlaus 1, Gunter Reuter 1, Peter Maroy 1, Janos Szidonya 1, Asa Rasmuson-Lestander 1, Karin Ekström 1, Barry Dickson 1, Christoph Hugentobler 1, Hugo Stocker 1, Ernst Hafen 1, Jean Antoine Lepesant 1, Gert Pflugfelder 1, Martin Heisenberg 1, Bernard Mechler 1, Florenci Serras 1, Montserrat Corominas 1, Stephan Schneuwly 1, Thomas Preat 1, John Roote 1, Steven Russell 1
PMCID: PMC1470913  PMID: 15238529

Abstract

We describe a collection of P-element insertions that have considerable utility for generating custom chromosomal aberrations in Drosophila melanogaster. We have mobilized a pair of engineered P elements, p[RS3] and p[RS5], to collect 3243 lines unambiguously mapped to the Drosophila genome sequence. The collection contains, on average, an element every 35 kb. We demonstrate the utility of the collection for generating custom chromosomal deletions that have their end points mapped, with base-pair resolution, to the genome sequence. The collection was generated in an isogenic strain, thus affording a uniform background for screens where sensitivity to genetic background is high. The entire collection, along with a computational and genetic toolbox for designing and generating custom deletions, is publicly available. Using the collection it is theoretically possible to generate >12,000 deletions between 1 bp and 1 Mb in size by simple eye color selection. In addition, a further 37,000 deletions, selectable by molecular screening, may be generated. We are now using the collection to generate a second-generation deficiency kit that is precisely mapped to the genome sequence.

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