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. 2004 Jul;167(3):1133–1142. doi: 10.1534/genetics.104.026260

A role for DNA polymerase delta in gene conversion and crossing over during meiosis in Saccharomyces cerevisiae.

Laurent Maloisel 1, Jaya Bhargava 1, G Shirleen Roeder 1
PMCID: PMC1470953  PMID: 15280229

Abstract

A screen for mutants of budding yeast defective in meiotic gene conversion identified a novel allele of the POL3 gene. POL3 encodes the catalytic subunit of DNA polymerase delta, an essential DNA polymerase involved in genomic DNA replication. The new allele, pol3-ct, specifies a protein missing the last four amino acids. pol3-ct shows little or no defect in DNA replication, but displays a reduction in the length of meiotic gene conversion tracts and a decrease in crossing over. We propose a model in which DNA synthesis determines the length of strand exchange intermediates and influences their resolution toward crossing over.

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Selected References

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