Abstract
A novel method for the deprotection of oligodeoxyribonucleotides under microwave irradiation has been developed. The oligodeoxynucleotides having base labile, phenoxyacetyl (pac), protection for exocyclic amino functions were fully deprotected in 0. 2 M sodium hydroxide (methanol:water : : 1:1, v/v) = A and 1 M sodium hydroxide (methanol:water : : 1:1, v/v) = B using microwaves in 4 and 2 min, respectively. The deprotection of oligodeoxyribonucleotides carrying conventional protecting groups, dAbz, dCbzand dGpac, for exocyclic amino functions was achieved in 4 min in B without any side product formation. The deprotected oligonucleotides were compared with the oligomers deprotected using standard deprotection conditions (29% aq. ammonia, 16 h, 55 degrees C) with respect to their retention time on HPLC and biological activity.
Full Text
The Full Text of this article is available as a PDF (56.9 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Boal J. H., Wilk A., Harindranath N., Max E. E., Kempe T., Beaucage S. L. Cleavage of oligodeoxyribonucleotides from controlled-pore glass supports and their rapid deprotection by gaseous amines. Nucleic Acids Res. 1996 Aug 1;24(15):3115–3117. doi: 10.1093/nar/24.15.3115. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pon R. T., Buck G. A., Niece R. L., Robertson M., Smith A. J., Spicer E. A survey of nucleic acid services in core laboratories. Biotechniques. 1994 Sep;17(3):526–534. [PubMed] [Google Scholar]
- Schulhof J. C., Molko D., Teoule R. Synthesis of DNA fragments containing 5,6-dihydrothymine, a major product of thymine gamma radiolysis. Nucleic Acids Res. 1988 Jan 11;16(1):319–326. doi: 10.1093/nar/16.1.319. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sinha N. D., Davis P., Usman N., Pérez J., Hodge R., Kremsky J., Casale R. Labile exocyclic amine protection of nucleosides in DNA, RNA and oligonucleotide analog synthesis facilitating N-deacylation, minimizing depurination and chain degradation. Biochimie. 1993;75(1-2):13–23. doi: 10.1016/0300-9084(93)90019-o. [DOI] [PubMed] [Google Scholar]