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Nucleic Acids Research logoLink to Nucleic Acids Research
. 1998 Jan 15;26(2):679–680. doi: 10.1093/nar/26.2.679

Efficient production of Cre-mediated site-directed recombinants through the utilization of the puromycin resistance gene, pac: a transient gene-integration marker for ES cells.

M Taniguchi 1, M Sanbo 1, S Watanabe 1, I Naruse 1, M Mishina 1, T Yagi 1
PMCID: PMC147263  PMID: 9421534

Abstract

Gene targeting in embryonic stem (ES) cells is a powerful tool for generating mice carrying specifically designed mutations in the germline. Puromycin can completely kill ES cells within 24 to 48 h whereas G418 and hygromycin cannot. We have, therefore, proposed that the puromycin N-acetyltransferase ( pac ) gene, may be utilized as a transient gene-integration marker. Using a circular expression vector of cre and pac genes, Cre-mediated mutant cells were effectively enriched by pulse treatment of puromycin without stable integration of their genes. We have thus demonstrated the first application of pac as a transient gene-integration marker for ES cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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