genetics. For the article “Human centromeric chromatin is a dynamic chromosomal domain that can spread over noncentromeric DNA,” by Ai Leen Lam, Christopher D. Boivin, Caitlin F. Bonney, M. Katharine Rudd, and Beth A. Sullivan, which appeared in issue 11, March 14, 2006, of Proc. Natl. Acad. Sci. USA (103, 4186–4191; first published March 6, 2006; 10.1073/pnas.0507947103), the authors note that the legend for Fig. 2 appeared incorrectly. The figure and its corrected legend appear below. This error does not affect the conclusions of the article.
Fig. 2.
CENP-A spreads over noncentromeric DNA on human artificial chromosomes. (A) Proposed models for sequence-dependent (Model 1) or sequence-independent (Model 2) assembly of CENP-A on human artificial chromosomes. (B–D) IF-FISH on individual chromatin fibers of DXZ1-derived human artificial chromosomes shows localization of CENP-A (blue), PAC vector DNA (red), and α-satellite DNA (green). Colored arrowheads show overlap between CENP-A/PAC DNA and CENP-A/α-satellite DNA. (Scale bar, 15 μm.) Arrowed lines denote CENP-A staining (blue), illustrating that CEN chromatin containing CENP-A and H3K4me2 is organized as a single domain and not as multiple blocks along the entire artificial chromosome.