Skip to main content
PMC home page
Biophysical Journal logoLink to Biophysical Journal
. 1977 Mar;17(3):255–267. doi: 10.1016/S0006-3495(77)85654-3

Spin-lattice relaxation times for 13C in isotope-enriched glycine accumulated in frog muscle.

M C Neville, H R Wyssbrod
PMCID: PMC1473242  PMID: 300254

Abstract

Spin-lattice relaxation times (T1's) of 13C-enriched glycine accumulated in frog muscles were determined at 1 degrees C by the inversion-recovery (180 degrees -tau-90 degree pulse sequence) method and compared with the values obtained in free solution. The value of T1 for the alpha-13C nucleus of glycine in the tissue was 50% of that obtained in free solution. The observed value for T1 in the tissue was not concentration-dependent, and no difference in chemical shift was observed between tissue and free solution. Quantification of the area under the glycine peak suggested that the observed signal represents at least 80% of the intracellular glycine. An average nuclear Overhauser enhancement of 2.83 for intracellular glycine indicates that the relaxation mechanism within the cell is predominantly dipolar, as in free solution. The value of T1 for the 13C' nucleus of glycine in the tissue was 67% of that in a solution of similar concentration. A quantitative analysis of the findings suggests that the observed difference in the value of T1 between tissue and free solution results from a difference in viscosity. The data provide no evidence either for special organization of intracellular water or for glycine binding. It is proposed that intracellular diffusion coefficients may be determined from measurements of 13C T1's of 13C-enriched intracellular solutes.

Full text

PDF
255

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abetsedarskaia L. A., Miftakhutdinova F. G., Fedotov V. D. O sostoianii vody v zhivykh tkaniakh (rezul'taty issledovanii metodom IaMR-spinovoe ékho) Biofizika. 1968 Jul-Aug;13(4):630–636. [PubMed] [Google Scholar]
  2. Armitage I. M., Huber H., Pearson H., Roberts J. D. Nuclear magnetic resonance spectroscopy. Carbon-13 spin-lattice relaxation time measurements of amino acids. Proc Natl Acad Sci U S A. 1974 May;71(5):2096–2097. doi: 10.1073/pnas.71.5.2096. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chang D. C., Rorschach H. E., Nichols B. L., Hazlewood C. F. Implications of diffusion coefficient measurements for the structure of cellular water. Ann N Y Acad Sci. 1973 Mar 30;204:434–443. doi: 10.1111/j.1749-6632.1973.tb30796.x. [DOI] [PubMed] [Google Scholar]
  4. Civan M. M., Shporer M. Pulsed nuclear magnetic resonance study of 17-O, 2-D, and 1-H of water in frog striated muscle. Biophys J. 1975 Apr;15(4):299–306. doi: 10.1016/S0006-3495(75)85820-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cooke R., Wien R. Nuclear magnetic resonance studies of intracellular water protons. Ann N Y Acad Sci. 1973 Mar 30;204:197–209. doi: 10.1111/j.1749-6632.1973.tb30780.x. [DOI] [PubMed] [Google Scholar]
  6. Finch E. D., Harmon J. F., Muller B. H. Pulsed NMR measurements of the diffusion constant of water in muscle. Arch Biochem Biophys. 1971 Nov;147(1):299–310. doi: 10.1016/0003-9861(71)90337-7. [DOI] [PubMed] [Google Scholar]
  7. Hazlewood C. F., Chang D. C., Nichols B. L., Woessner D. E. Nuclear magnetic resonance transverse relaxation times of water protons in skeletal muscle. Biophys J. 1974 Aug;14(8):583–606. doi: 10.1016/S0006-3495(74)85937-0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kushmerick M. J., Podolsky R. J. Ionic mobility in muscle cells. Science. 1969 Dec 5;166(3910):1297–1298. doi: 10.1126/science.166.3910.1297. [DOI] [PubMed] [Google Scholar]
  9. Neville M. C. Amino acid accumulation in frog muscle. II. Are cycloleucine fluxes consistent with an adsorption model for concentrative uptake of amino acid? Biochim Biophys Acta. 1975 Mar 25;382(3):393–409. doi: 10.1016/0005-2736(75)90280-1. [DOI] [PubMed] [Google Scholar]
  10. Neville M. C. Cellular accumulation of amino acids: adsorption revisited. Ann N Y Acad Sci. 1973 Mar 30;204:538–563. doi: 10.1111/j.1749-6632.1973.tb30803.x. [DOI] [PubMed] [Google Scholar]
  11. Outhred R. K., George E. P. Water and ions in muscles and model systems. Biophys J. 1973 Feb;13(2):97–103. doi: 10.1016/S0006-3495(73)85972-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Walter J. A., Hope A. B. Nuclear magnetic resonance and the state of water in cells. Prog Biophys Mol Biol. 1971;23:1–20. doi: 10.1016/0079-6107(71)90015-0. [DOI] [PubMed] [Google Scholar]

Articles from Biophysical Journal are provided here courtesy of The Biophysical Society

RESOURCES