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. 1998 Feb 15;26(4):1130–1131. doi: 10.1093/nar/26.4.1130

Efficient reamplification of differential display products by transient ligation and thermal asymmetric PCR.

S Bonnet 1, G Prévot 1, C Bourgouin 1
PMCID: PMC147339  PMID: 9461480

Abstract

A new method for specific reamplification of DDRT-PCR products is presented. After transient ligation of the primary DDRT-PCR fragments into a T-vector, the cDNAs of interest were reamplified by hemi-nested PCR and thermally asymmetric cycles. In contrast to the originally described protocol, this method of reamplification is specific, sensitive, reproducibly gives a high yield of DNA and allows direct sequencing of the reamplified product without purification or cloning.

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Selected References

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