Abstract
A method is described for the first time for rapid and accurate discrimination among several algal types by their light-scattering properties alone. Using a multiangle light-scattering flow system, we obtained light-scatter patterns for individual cells in asynchronous cultures of Chlorella, Chlamydomonas, and Anacystis. The patterns are consistent and distinct for each species. By these signatures, each algal type can be recognized within mixtures.
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Selected References
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- Chiang K. S., Sueoka N. Replication of chloroplast DNA in Chlamydomonas reinhardi during vegetative cell cycle: its mode and regulation. Proc Natl Acad Sci U S A. 1967 May;57(5):1506–1513. doi: 10.1073/pnas.57.5.1506. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kollman V. H., Shapiro S. L., Campillo A. J. Photosynthetic studies with a 10-psec resolution streak camera. Biochem Biophys Res Commun. 1975 Apr 21;63(4):917–923. doi: 10.1016/0006-291x(75)90656-7. [DOI] [PubMed] [Google Scholar]
- Meselson M., Stahl F. W. THE REPLICATION OF DNA IN ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1958 Jul 15;44(7):671–682. doi: 10.1073/pnas.44.7.671. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mullaney P. F., Van Dilla M. A., Coulter J. R., Dean P. N. Cell sizing: a light scattering photometer for rapid volume determination. Rev Sci Instrum. 1969 Aug;40(8):1029–1032. doi: 10.1063/1.1684143. [DOI] [PubMed] [Google Scholar]
- Salzman G. C., Crowell J. M., Goad C. A., Hansen K. M., Hiebert R. D., LaBauve P. M., Martin J. C., Ingram M. L., Mullaney P. F. A flow-system multiangle light-scattering instrument for cell characterization. Clin Chem. 1975 Aug;21(9):1297–1304. [PubMed] [Google Scholar]
- Steinkamp J. A., Fulwyler M. J., Coulter J. R., Hiebert R. D., Horney J. L., Mullancy P. F. A new multiparameter separator for microscopic particles and biological cells. Rev Sci Instrum. 1973 Sep;44(9):1301–1310. doi: 10.1063/1.1686375. [DOI] [PubMed] [Google Scholar]