Abstract
The photoelectric activity of different parts of lipid bilayer containing bacteriorhodopsin was investigated by moving a small actinic light spot across the Plateau-Gibbs border and the bimolecular part of this reconstituted model membrane. The results give direct evidence that bacteriorhodopsin incorporated into the bimolecular region of the lipid membrane is responsible for the photoelectric activity of this system. A technique for scanning the photoelectric activity of a modified bimolecular lipid membrane is described in detail.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Dancsházy Z., Karvaly B. Incorporation of bacteriorhodopsin into a bilayer lipid membrane; a photoelectric-spectroscopic study. FEBS Lett. 1976 Dec 15;72(1):136–138. doi: 10.1016/0014-5793(76)80829-0. [DOI] [PubMed] [Google Scholar]
- Drachev L. A., Frolov V. N., Kaulen A. D., Liberman E. A., Ostroumov S. A., Plakunova V. G., Semenov A. Y., Skulachev V. P. Reconstitution of Biological Molecular generators of electric current. Bacteriorhodopsin. J Biol Chem. 1976 Nov 25;251(22):7059–7065. [PubMed] [Google Scholar]
- Hartmann W., Galla H. J., Sackmann E. Direct evidence of charge-induced lipid domain structure in model membranes. FEBS Lett. 1977 Jun 15;78(2):169–172. doi: 10.1016/0014-5793(77)80298-6. [DOI] [PubMed] [Google Scholar]
- Hong F. T., Mauzerall D. Photoemf at a single membrane-solution interface specific to lipid bilayers containing magnesium porphyrins. Nat New Biol. 1972 Nov 29;240(100):154–155. doi: 10.1038/newbio240154a0. [DOI] [PubMed] [Google Scholar]
- Ormos P., Dancsházy Z., Karvaly B. Mechanism of generation and regulation of photopotential by bacteriorhodopsin in bimolecular lipid membrane. Biochim Biophys Acta. 1978 Aug 8;503(2):304–315. doi: 10.1016/0005-2728(78)90190-1. [DOI] [PubMed] [Google Scholar]
- Shieh P., Packer L. Photo-induced potentials across a polymer stabilized planar membrane, in the presence of bacteriorhodopsin. Biochem Biophys Res Commun. 1976 Jul 26;71(2):603–609. doi: 10.1016/0006-291x(76)90830-5. [DOI] [PubMed] [Google Scholar]