Abstract
Using the simple argument based on irreversible thermodynamics and the Gouy-Chapman theory of the double layer, we show that the equilibrium distribution of charged lipid molecules between the two surfaces of a bilayer is asymmetric if the two solutions bathing the surfaces have the same ionic strength but contain ions of different valencies. For example, if one bathing solution contains 0.10 M NaCl and the other contains 0.70 M NaCl and 0.10 M CaCl2, the ratio of charged lipid molecules of the two surfaces in a membrane that contains 50% total negative lipids is 1.46, leading to a transbilayer potential of 18 mV. A complete set of such numerical results is presented in four figures.
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Selected References
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- Ehrenstein G., Gilbert D. L., Lipicky R. J. Does phospholipid flip-flop affect axon potassium channels? Biophys J. 1975 Aug;15(8):847–849. doi: 10.1016/S0006-3495(75)85859-0. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Israelachvili J. N. Theoretical considerations on the asymmetric distribution of charged phospholipid molecules on the inner and outer layers of curved bilayer membranes. Biochim Biophys Acta. 1973 Nov 16;323(4):659–663. doi: 10.1016/0005-2736(73)90179-x. [DOI] [PubMed] [Google Scholar]
- McLaughlin S., Harary H. Phospholipid flip-flop and the distribution of surface charges in excitable membranes. Biophys J. 1974 Mar;14(3):200–208. doi: 10.1016/S0006-3495(74)85907-2. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nelson A. P., Colonomos P., McQuarrie D. A. Electrostatic coupling across a membrane with titratable surface groups. J Theor Biol. 1975 Apr;50(2):317–325. doi: 10.1016/0022-5193(75)90084-3. [DOI] [PubMed] [Google Scholar]
- Olivares W., McQuarrie D. A. On the theory of ionic solutions. Biophys J. 1975 Feb;15(2 Pt 1):143–162. doi: 10.1016/s0006-3495(75)85798-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sherwood D., Montal M. Transmembrane lipid migration in planar asymmetric bilayer membranes. Biophys J. 2009 Jan 1;15(5):417–434. doi: 10.1016/S0006-3495(75)85827-9. [DOI] [PMC free article] [PubMed] [Google Scholar]