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. 1978 Sep;23(3):395–405. doi: 10.1016/S0006-3495(78)85458-7

Fluorescein excitation and emission polarization spectra in living cells: changes during the cell cycle.

L Cercek, B Cercek, C H Ockey
PMCID: PMC1473529  PMID: 698344

Abstract

Changes in the fluorescein fluorescence emission and excitation polarization spectra in synchronized cultured S3 fibroblasts at G1, mid-S, and mitosis, as well as in human lymphocytes before and after stimulation with mitogens, were studied. In contrast to those measured in aqueous solutions the emission and excitation polarization spectra in living cells exhibit a wavelength dependence characteristic for the state of the cell cycle. Changes in the temperature and in the amount of intracellular water result in quantitative wavelength-independent changes in the polarization spectra. Possible mechanisms for the qualitative wavelength-dependent changes in the fluorescein emission and excitation polarization spectra during the cell cycle are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CHEN R. F., BOWMAN R. L. FLUORESCENCE POLARIZATION: MEASUREMENT WITH ULTRAVIOLET-POLARIZING FILTERS IN A SPECTROPHOTOFLUOROMETER. Science. 1965 Feb 12;147(3659):729–732. doi: 10.1126/science.147.3659.729. [DOI] [PubMed] [Google Scholar]
  2. Cercek L., Cercek B. Application of the phenomenon of changes in the structuredness of cytoplasmic matrix (SCM) in the diagnosis of malignant disorders: a review. Eur J Cancer. 1977 Sep;13(9):903–915. doi: 10.1016/0014-2964(77)90166-9. [DOI] [PubMed] [Google Scholar]
  3. Cercek L., Cercek B. Changes in the structuredness of cytoplasmic matrix (SCM) in human lymphocytes induced by PHA and cancer basic protein as measured in single cells. Br J Cancer. 1976 May;33(5):539–543. doi: 10.1038/bjc.1976.85. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cercek L., Cercek B. Effects of osmomolarity, calcium and magnesium ions on the structuredness of cytoplasmic matrix (SCM). Radiat Environ Biophys. 1976 Mar 30;13(1):9–12. doi: 10.1007/BF01323618. [DOI] [PubMed] [Google Scholar]
  5. Cercek L., Cercek B., Ockey C. H. Structuredness of the cytoplasmic matrix and Michaelis-Menten constants for the hydrolysis of FDA during the cell cycle in Chinese hamster ovary cells. Biophysik. 1973;10(3):187–194. doi: 10.1007/BF01190577. [DOI] [PubMed] [Google Scholar]
  6. Cercek L., Cercek B. Relationship between changes in the structuredness of cytoplasm and rate constants for the hydrolysis of FDA in Saccharomyces cerevisiae. Biophysik. 1973;9(2):109–112. doi: 10.1007/BF01215849. [DOI] [PubMed] [Google Scholar]
  7. Cercek L., Milenkovic P., Cercek B., Lajtha L. G. Induction of PHA response in mouse bone marrow cells by thymic extracts as studied by changes in the structuredness of cytoplasmic matrix. Immunology. 1975 Nov;29(5):885–891. [PMC free article] [PubMed] [Google Scholar]
  8. Harris R., Ukaejiofo E. O. Rapid preparation of lymphocytes for tissue-typing. Lancet. 1969 Aug 9;2(7615):327–327. doi: 10.1016/s0140-6736(69)90096-8. [DOI] [PubMed] [Google Scholar]
  9. Lindmo T., Steen H. B. Flow cytometric measurement of the polarization of fluorescence from intracellular fluorescein in mammalian cells. Biophys J. 1977 May;18(2):173–187. doi: 10.1016/S0006-3495(77)85606-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lord B. I., Cercek L., Cercek B., Shah G. P., Dexter T. M., Lajtha L. G. Inhibitors of haemopoietic cell proliferation? Specificity of action within the haemopoietic system. Br J Cancer. 1974 Feb;29(2):168–175. doi: 10.1038/bjc.1974.53. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Rotman B., Papermaster B. W. Membrane properties of living mammalian cells as studied by enzymatic hydrolysis of fluorogenic esters. Proc Natl Acad Sci U S A. 1966 Jan;55(1):134–141. doi: 10.1073/pnas.55.1.134. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sachs F., Latorre R. Cytoplasmic solvent structure of single barnacle muscle cells studied by electron spin resonance. Biophys J. 1974 Apr;14(4):316–326. doi: 10.1016/S0006-3495(74)85918-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. WEBER G. Polarization of the fluorescence of macromolecules. I. Theory and experimental method. Biochem J. 1952 May;51(2):145–155. doi: 10.1042/bj0510145. [DOI] [PMC free article] [PubMed] [Google Scholar]

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