Abstract
A Ca-sensitive dye, arsenazo III, has been incorporated into resealed human erythrocyte ghosts and calibrated to monitor continuously micromolar concentrations of intracellular ionized Ca ([Ca++]i). When the external concentration of Ca is much greater than [Ca++]i, [Ca++]i increases because of a net balance between Ca influx and efflux. Dynamic changes in [Ca++]i regulate K efflux, which in turn may influence the rate of Ca influx. A procedure for purifying arsenazo III is also described.
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Selected References
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- Blum R. M., Hoffman J. F. Ca-induced K transport in human red cells: localization of the Ca-sensitive site to the inside of the membrane. Biochem Biophys Res Commun. 1972 Feb 16;46(3):1146–1152. doi: 10.1016/s0006-291x(72)80094-9. [DOI] [PubMed] [Google Scholar]
- Bodemann H., Passow H. Factors controlling the resealing of the membrane of human erythrocyte ghosts after hypotonic hemolysis. J Membr Biol. 1972;8(1):1–26. doi: 10.1007/BF01868092. [DOI] [PubMed] [Google Scholar]
- Brown J. E., Brown P. K., Pinto L. H. Detection of light-induced changes of intracellular ionized calcium concentration in Limulus ventral photoreceptors using arsenazo III. J Physiol. 1977 May;267(2):299–320. doi: 10.1113/jphysiol.1977.sp011814. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brown J. E., Cohen L. B., De Weer P., Pinto L. H., Ross W. N., Salzberg B. M. Rapid changes in intracellular free calcium concentration. Detection by metallochromic indicator dyes in squid giant axon. Biophys J. 1975 Nov;15(11):1155–1160. doi: 10.1016/S0006-3495(75)85891-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dipolo R., Requena J., Brinley F. J., Jr, Mullins L. J., Scarpa A., Tiffert T. Ionized calcium concentrations in squid axons. J Gen Physiol. 1976 Apr;67(4):433–467. doi: 10.1085/jgp.67.4.433. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HOFFMAN J. F. Cation transport and structure of the red-cell plasma membrane. Circulation. 1962 Nov;26:1202–1213. doi: 10.1161/01.cir.26.5.1201. [DOI] [PubMed] [Google Scholar]
- Harrison D. G., Long C. The calcium content of human erythrocytes. J Physiol. 1968 Dec;199(2):367–381. doi: 10.1113/jphysiol.1968.sp008658. [DOI] [PMC free article] [PubMed] [Google Scholar]
- JORGENSEN S., OVERGAARD-HANSEN K., PRAETORIUS E. Rephosphorylation produced by inosine and adenosine of adenosine monophosphate and adenosine diphosphate in human erythrocytes. Nature. 1957 Jan 19;179(4551):152–153. doi: 10.1038/179152a0. [DOI] [PubMed] [Google Scholar]
- Kendrick N. C. Purification of arsenazo III, a Ca2+-sensitive dye. Anal Biochem. 1976 Dec;76(2):487–501. doi: 10.1016/0003-2697(76)90342-0. [DOI] [PubMed] [Google Scholar]
- Knauf P. A., Riordan J. R., Schuhmann B., Wood-Guth I., Passow H. Calcium-potassium-stimulated net potassium efflux from human erythrocyte ghosts. J Membr Biol. 1975 Dec 4;25(1-2):1–22. doi: 10.1007/BF01868565. [DOI] [PubMed] [Google Scholar]
- Kregenow F. M., Hoffman J. F. Some kinetic and metabolic characteristics of calcium-induced potassium transport in human red cells. J Gen Physiol. 1972 Oct;60(4):406–429. doi: 10.1085/jgp.60.4.406. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lew V. L., Ferreira H. G. Variable Ca sensitivity of a K-selective channel in intact red-cell membranes. Nature. 1976 Sep 23;263(5575):336–338. doi: 10.1038/263336a0. [DOI] [PubMed] [Google Scholar]
- Miledi R., Parker I., Schalow G. Measurement of calcium transients in frog muscle by the use of arsenazo III. Proc R Soc Lond B Biol Sci. 1977 Aug 22;198(1131):201–210. doi: 10.1098/rspb.1977.0094. [DOI] [PubMed] [Google Scholar]
- Porzig H. Studies on the cation permeability of human red cell ghosts. Characterization and biological significance of two membrane sites with high affinities for Ca. J Membr Biol. 1977 Mar 23;31(4):317–349. doi: 10.1007/BF01869411. [DOI] [PubMed] [Google Scholar]
- Sarkadi B., Szász I., Gerlóczy A., Gárdos G. Transport parameters and stoichiometry of active calcium ion extrusion in intact human red cells. Biochim Biophys Acta. 1977 Jan 4;464(1):93–107. doi: 10.1016/0005-2736(77)90373-x. [DOI] [PubMed] [Google Scholar]
- Schatzmann H. J. Dependence on calcium concentration and stoichiometry of the calcium pump in human red cells. J Physiol. 1973 Dec;235(2):551–569. doi: 10.1113/jphysiol.1973.sp010403. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schatzmann H. J., Vincenzi F. F. Calcium movements across the membrane of human red cells. J Physiol. 1969 Apr;201(2):369–395. doi: 10.1113/jphysiol.1969.sp008761. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Simons T. J. Calcium-dependent potassium exchange in human red cell ghosts. J Physiol. 1976 Mar;256(1):227–244. doi: 10.1113/jphysiol.1976.sp011322. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thomas M. V., Gorman A. L. Internal calcium changes in a bursting pacemaker neuron measured with arsenazo III. Science. 1977 Apr 29;196(4289):531–533. doi: 10.1126/science.850795. [DOI] [PubMed] [Google Scholar]
