Abstract
Postreplication repair in synchronous Chinese hamster cells was determined after split doses of ultraviolet (UV) radiation. Repair was enhanced by irradiation of cells in G2 or S-phase with a small dose of UV radiation at least 1.5 h before a three-fold larger dose of UV. There was significantly greater enhancement when the first dose was given in G2 than when it was given in the S-phase 0.5-1.5 h before the test dose. These data indicate that enhancement of postreplication repair does not require active DNA replication and qualitatively is independent of when in the cell cycle the cells are irradiated.
Full text
PDF







Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Aebersold P. M., Burki H. J. 5-bromodeoxyuridine mutagenesis in synchronous hamster cells. Mutat Res. 1976 Jan;40(1):63–66. doi: 10.1016/0165-1218(76)90024-0. [DOI] [PubMed] [Google Scholar]
- Buhl S. N., Setlow R. B., Regan J. D. Recovery of the ability to synthesize DNA in segments of normal size at long times after ultraviolet irradiation of human cells. Biophys J. 1973 Dec;13(12):1265–1275. doi: 10.1016/S0006-3495(73)86061-8. [DOI] [PMC free article] [PubMed] [Google Scholar]
- D'Ambrosio S. M., Setlow R. B. Defective and enhanced postreplication repair in classical and variant xeroderma pigmentosum cells treated with N-acetoxy-2-acetylaminofluorene. Cancer Res. 1978 Apr;38(4):1147–1153. [PubMed] [Google Scholar]
- D'Ambrosio S. M., Setlow R. B. Enhancement of postreplication repair in Chinese hamster cells. Proc Natl Acad Sci U S A. 1976 Jul;73(7):2396–2400. doi: 10.1073/pnas.73.7.2396. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Elkind M. M., Kamper C. Two forms of repair of DNA in mammalian cells following irradiation. Biophys J. 1970 Mar;10(3):237–245. doi: 10.1016/S0006-3495(70)86296-8. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fujiwara Y., Tatsumi M. Replicative bypass repair of ultraviolet damage to DNA of mammalian cells: caffeine sensitive and caffeine resistant mechanisms. Mutat Res. 1976 Oct;37(1):91–110. doi: 10.1016/0027-5107(76)90058-0. [DOI] [PubMed] [Google Scholar]
- Han A., Sinclair W. K. Sensitivity of synchronized Chinese hamster cells to ultraviolet light. Biophys J. 1969 Sep;9(9):1171–1192. doi: 10.1016/S0006-3495(69)86444-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hart R. W., Setlow R. B. Correlation between deoxyribonucleic acid excision-repair and life-span in a number of mammalian species. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2169–2173. doi: 10.1073/pnas.71.6.2169. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Higgins N. P., Kato K., Strauss B. A model for replication repair in mammalian cells. J Mol Biol. 1976 Mar 5;101(3):417–425. doi: 10.1016/0022-2836(76)90156-x. [DOI] [PubMed] [Google Scholar]
- Meyn R. E., Kasschau M. R., Hewitt R. R. The recovery of normal DNA replication kinetics in ultraviolet-irradiated Chinese hamster cells. Mutat Res. 1977 Jul;44(1):129–138. doi: 10.1016/0027-5107(77)90121-x. [DOI] [PubMed] [Google Scholar]
- Setlow R. B., Regan J. D., German J., Carrier W. L. Evidence that xeroderma pigmentosum cells do not perform the first step in the repair of ultraviolet damage to their DNA. Proc Natl Acad Sci U S A. 1969 Nov;64(3):1035–1041. doi: 10.1073/pnas.64.3.1035. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sinclair W. K. Hydroxyurea: effects on Chinese hamster cells grown in culture. Cancer Res. 1967 Feb;27(2):297–308. [PubMed] [Google Scholar]
- Strauss B. S. Repair of DNA in mammalian cells. Life Sci. 1974 Nov 15;15(10):1685–1693. doi: 10.1016/0024-3205(74)90171-4. [DOI] [PubMed] [Google Scholar]
- TROSKO J. E., CHU E. H., CARRIER W. L. THE INDUCTION OF THYMINE DIMERS IN ULTRAVIOLET-IRRADIATED MAMMALIAN CELLS. Radiat Res. 1965 Apr;24:667–672. [PubMed] [Google Scholar]
- Tobey R. A., Anderson E. C., Petersen D. F. Properties of mitotic cells prepared by mechanically shaking monolayer cultures of Chinese hamster cells. J Cell Physiol. 1967 Aug;70(1):63–68. doi: 10.1002/jcp.1040700109. [DOI] [PubMed] [Google Scholar]
- Todd P. Fractionated ultraviolet light irradiation of cultured Chinese hamster cells. Radiat Res. 1973 Jul;55(1):93–100. [PubMed] [Google Scholar]
