Skip to main content
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1998 Mar 15;26(6):1544–1545. doi: 10.1093/nar/26.6.1544

In vivo mapping of nucleosomes using psoralen-DNA crosslinking and primer extension.

R E Wellinger 1, J M Sogo 1
PMCID: PMC147415  PMID: 9490804

Abstract

By the use of psoralen crosslinking and primer extension, a method was developed which allows the analysis of chromatin structure in vivo. Using a yeast minichromosome, >9 nucleosomes were mapped with a resolution of at least +/-30 bp.

Full Text

The Full Text of this article is available as a PDF (190.2 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Conconi A., Losa R., Koller T., Sogo J. M. Psoralen-crosslinking of soluble and of H1-depleted soluble rat liver chromatin. J Mol Biol. 1984 Oct 5;178(4):920–928. doi: 10.1016/0022-2836(84)90319-x. [DOI] [PubMed] [Google Scholar]
  2. Conconi A., Sogo J. M., Ryan C. A. Ribosomal gene clusters are uniquely proportioned between open and closed chromatin structures in both tomato leaf cells and exponentially growing suspension cultures. Proc Natl Acad Sci U S A. 1992 Jun 15;89(12):5256–5260. doi: 10.1073/pnas.89.12.5256. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gale J. M., Smerdon M. J. UV-induced pyrimidine dimers and trimethylpsoralen cross-links do not alter chromatin folding in vitro. Biochemistry. 1988 Sep 20;27(19):7197–7205. doi: 10.1021/bi00419a006. [DOI] [PubMed] [Google Scholar]
  4. Hanson C. V., Shen C. K., Hearst J. E. Cross-linking of DNA in situ as a probe for chromatin structure. Science. 1976 Jul 2;193(4247):62–64. doi: 10.1126/science.935855. [DOI] [PubMed] [Google Scholar]
  5. Lilley D. M. Probes of DNA structure. Methods Enzymol. 1992;212:133–139. doi: 10.1016/0076-6879(92)12009-f. [DOI] [PubMed] [Google Scholar]
  6. Sogo J. M., Thoma F. Electron microscopy of chromatin. Methods Enzymol. 1989;170:142–165. doi: 10.1016/0076-6879(89)70045-8. [DOI] [PubMed] [Google Scholar]
  7. Tanaka S., Livingstone-Zatchej M., Thoma F. Chromatin structure of the yeast URA3 gene at high resolution provides insight into structure and positioning of nucleosomes in the chromosomal context. J Mol Biol. 1996 Apr 19;257(5):919–934. doi: 10.1006/jmbi.1996.0212. [DOI] [PubMed] [Google Scholar]
  8. Thoma F. Protein-DNA interactions and nuclease-sensitive regions determine nucleosome positions on yeast plasmid chromatin. J Mol Biol. 1986 Jul 20;190(2):177–190. doi: 10.1016/0022-2836(86)90291-3. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES