Abstract
By the use of psoralen crosslinking and primer extension, a method was developed which allows the analysis of chromatin structure in vivo. Using a yeast minichromosome, >9 nucleosomes were mapped with a resolution of at least +/-30 bp.
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Selected References
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- Conconi A., Losa R., Koller T., Sogo J. M. Psoralen-crosslinking of soluble and of H1-depleted soluble rat liver chromatin. J Mol Biol. 1984 Oct 5;178(4):920–928. doi: 10.1016/0022-2836(84)90319-x. [DOI] [PubMed] [Google Scholar]
- Conconi A., Sogo J. M., Ryan C. A. Ribosomal gene clusters are uniquely proportioned between open and closed chromatin structures in both tomato leaf cells and exponentially growing suspension cultures. Proc Natl Acad Sci U S A. 1992 Jun 15;89(12):5256–5260. doi: 10.1073/pnas.89.12.5256. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gale J. M., Smerdon M. J. UV-induced pyrimidine dimers and trimethylpsoralen cross-links do not alter chromatin folding in vitro. Biochemistry. 1988 Sep 20;27(19):7197–7205. doi: 10.1021/bi00419a006. [DOI] [PubMed] [Google Scholar]
- Hanson C. V., Shen C. K., Hearst J. E. Cross-linking of DNA in situ as a probe for chromatin structure. Science. 1976 Jul 2;193(4247):62–64. doi: 10.1126/science.935855. [DOI] [PubMed] [Google Scholar]
- Lilley D. M. Probes of DNA structure. Methods Enzymol. 1992;212:133–139. doi: 10.1016/0076-6879(92)12009-f. [DOI] [PubMed] [Google Scholar]
- Sogo J. M., Thoma F. Electron microscopy of chromatin. Methods Enzymol. 1989;170:142–165. doi: 10.1016/0076-6879(89)70045-8. [DOI] [PubMed] [Google Scholar]
- Tanaka S., Livingstone-Zatchej M., Thoma F. Chromatin structure of the yeast URA3 gene at high resolution provides insight into structure and positioning of nucleosomes in the chromosomal context. J Mol Biol. 1996 Apr 19;257(5):919–934. doi: 10.1006/jmbi.1996.0212. [DOI] [PubMed] [Google Scholar]
- Thoma F. Protein-DNA interactions and nuclease-sensitive regions determine nucleosome positions on yeast plasmid chromatin. J Mol Biol. 1986 Jul 20;190(2):177–190. doi: 10.1016/0022-2836(86)90291-3. [DOI] [PubMed] [Google Scholar]