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. 1987 Apr;71:139–146. doi: 10.1289/ehp.8771139

Normal versus abnormal structure: considerations in morphologic responses of teleosts to pollutants.

D E Hinton, R C Lantz, J A Hampton, P R McCuskey, R S McCuskey
PMCID: PMC1474344  PMID: 3297656

Abstract

Consideration of newer more quantitative morphologic approaches to the study of aquatic pollutants can provide opportunity for collaborative/integrated studies with other subdisciplines in toxicology. Current commonly employed morphologic approaches result largely in subjective findings difficult to analyze statistically and often are directed at levels of structural organization inconsistent with biochemical and physiological approaches. We review some of the methods and approaches available for correlated structure/function studies and present examples from normal and altered skin, gill, and liver of teleosts.

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Selected References

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  1. Belloni A. S., Mazzocchi G., Robba C., Gambino A. M., Nussdorfer G. G. An ultrastructural, morphometric and autoradiographic study of the effects of 7,12-dimethylbenzanthracene on the rat adrenal cortex. Virchows Arch B Cell Pathol. 1978 Jan 18;26(3):195–214. doi: 10.1007/BF02889548. [DOI] [PubMed] [Google Scholar]
  2. Bolender R. P. Correlation of morphometry and stereology with biochemical analysis of cell fractions. Int Rev Cytol. 1978;55:247–289. doi: 10.1016/s0074-7696(08)61890-2. [DOI] [PubMed] [Google Scholar]
  3. Bolender R. P. Stereology: applications to pharmacology. Annu Rev Pharmacol Toxicol. 1981;21:549–573. doi: 10.1146/annurev.pa.21.040181.003001. [DOI] [PubMed] [Google Scholar]
  4. ELIAS H., BENGELSDORF H. The structure of the liver of vertebrates. Acta Anat (Basel) 1952;14(4):297–337. doi: 10.1159/000140715. [DOI] [PubMed] [Google Scholar]
  5. Griffiths G., Warren G., Quinn P., Mathieu-Costello O., Hoppeler H. Density of newly synthesized plasma membrane proteins in intracellular membranes. I. Stereological studies. J Cell Biol. 1984 Jun;98(6):2133–2141. doi: 10.1083/jcb.98.6.2133. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hacking M. A., Budd J., Hodson K. The ultrastructure of the liver of the rainbow trout: normal structure and modifications after chronic administration of a polychlorinated biphenyl Aroclor 1254. Can J Zool. 1978 Mar;56(3):477–491. doi: 10.1139/z78-068. [DOI] [PubMed] [Google Scholar]
  7. Hampton J. A., McCuskey P. A., McCuskey R. S., Hinton D. E. Functional units in rainbow trout (Salmo gairdneri) liver: I. Arrangement and histochemical properties of hepatocytes. Anat Rec. 1985 Oct;213(2):166–175. doi: 10.1002/ar.1092130208. [DOI] [PubMed] [Google Scholar]
  8. Hawkes J. W. The effects of xenobiotics on fish tissues: morphological studies. Fed Proc. 1980 Dec;39(14):3230–3236. [PubMed] [Google Scholar]
  9. Huges G. M., Perry S. F. Morphometric study of trout gills: a light-microscopic method suitable for the evaluation of pollutant action. J Exp Biol. 1976 Apr;64(2):447–460. doi: 10.1242/jeb.64.2.447. [DOI] [PubMed] [Google Scholar]
  10. Iozzo R. V., Bolender R. P., Wight T. N. Proteoglycan changes in the intercellular matrix of human colon carcinoma: an integrated biochemical and stereologic analysis. Lab Invest. 1982 Aug;47(2):124–138. [PubMed] [Google Scholar]
  11. Klaunig J. E., Lipsky M. M., Trump B. F., Hinton D. E. Biochemical and ultrastructural changes in teleost liver following subacute exposure to PCB. J Environ Pathol Toxicol. 1979 Mar-Apr;2(4):953–963. [PubMed] [Google Scholar]
  12. Loud A. V., Anversa P. Morphometric analysis of biologic processes. Lab Invest. 1984 Mar;50(3):250–261. [PubMed] [Google Scholar]
  13. McCuskey R. S. A dynamic and static study of hepatic arterioles and hepatic sphincters. Am J Anat. 1966 Nov;119(3):455–477. doi: 10.1002/aja.1001190307. [DOI] [PubMed] [Google Scholar]
  14. McCuskey R. S., Urbaschek R., McCuskey P. A., Urbaschek B. In vivo microscopic observations of the responses of Kupffer cells and the hepatic microcirculation to Mycobacterium bovis BCG alone and in combination with endotoxin. Infect Immun. 1983 Oct;42(1):362–367. doi: 10.1128/iai.42.1.362-367.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. McCuskey R. S., Vonnahme F. J., Grün M. In vivo and electron microscopic observations of the hepatic microvasculature in the rat following portacaval anastomosis. Hepatology. 1983 Jan-Feb;3(1):96–104. doi: 10.1002/hep.1840030116. [DOI] [PubMed] [Google Scholar]
  16. McDowell E. M., Becci P. J., Schürch W., Trump B. F. The respiratory epithelium. VII. Epidermoid metaplasia of hamster tracheal epithelium during regeneration following mechanical injury. J Natl Cancer Inst. 1979 Apr;62(4):995–1008. [PubMed] [Google Scholar]
  17. Pfeifer K. F., Weber L. J., Larson R. E. Carbon tetrachloride-induced hepatotoxic response in rainbow trout, Salmo gairderni, as influenced by two commercial fish diets. Comp Biochem Physiol C. 1980;67C(2):91–96. doi: 10.1016/0306-4492(80)90002-7. [DOI] [PubMed] [Google Scholar]
  18. Pfeifer K. F., Weber L. J. The effect of carbon tetrachloride treatment on urine flow rate of the rainbow trout, Salmo gairdneri. Toxicol Appl Pharmacol. 1980 Feb;52(2):347–350. doi: 10.1016/0041-008x(80)90121-0. [DOI] [PubMed] [Google Scholar]
  19. Quinn P., Griffiths G., Warren G. Density of newly synthesized plasma membrane proteins in intracellular membranes II. Biochemical studies. J Cell Biol. 1984 Jun;98(6):2142–2147. doi: 10.1083/jcb.98.6.2142. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rappaport A. M. The microcirculatory acinar concept of normal and pathological hepatic structure. Beitr Pathol. 1976 May;157(3):215–243. doi: 10.1016/s0005-8165(76)80083-2. [DOI] [PubMed] [Google Scholar]
  21. Rees K. R., Tarlow M. J. The hepatotoxic action of allyl formate. Biochem J. 1967 Sep;104(3):757–761. doi: 10.1042/bj1040757. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Rohr H., Oberholzer M., Bartsch G., Keller M. Morphometry in experimental pathology: methods, baseline data and applications. Int Rev Exp Pathol. 1976;15:233–325. [PubMed] [Google Scholar]
  23. Scarpelli D. G. Drug metabolism and aflatoxin-induced hepatoma in rainbow trout (Salmo gairdneri). Prog Exp Tumor Res. 1976;20:339–350. doi: 10.1159/000398709. [DOI] [PubMed] [Google Scholar]
  24. Schoor W. P., Couch J. A. Correlation of mixed-function oxidase activity with ultrastructural changes in the liver of a marine fish. Cancer Biochem Biophys. 1979;4(2):95–103. [PubMed] [Google Scholar]
  25. Schwerdtfeger W. K. Morphometrical studies of the ultrastructure of the epidermis of the guppy, Poecilia reticulata Peters, following adaptation to seawater and treatment with prolactin. Gen Comp Endocrinol. 1979 Aug;38(4):476–483. doi: 10.1016/0016-6480(79)90156-4. [DOI] [PubMed] [Google Scholar]
  26. Schwerdtfeger W. K. Qualitative and quantitative data on the fine structure of the guppy (Poecilia reticulata Peters) epidermis following treatment with thyroxine and testosterone. Gen Comp Endocrinol. 1979 Aug;38(4):484–490. doi: 10.1016/0016-6480(79)90157-6. [DOI] [PubMed] [Google Scholar]
  27. Schär M., Maly I. P., Sasse D. Histochemical studies on metabolic zonation of the liver in the trout (Salmo gairdneri). Histochemistry. 1985;83(2):147–151. doi: 10.1007/BF00495145. [DOI] [PubMed] [Google Scholar]
  28. Shore T. W., Jones H. L. On the Structure of the Vertebrate Liver. J Physiol. 1889 Jul;10(5):408–432.24. doi: 10.1113/jphysiol.1889.sp000309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Smith R. J. Effects of 17 alpha-methyltestosterone on the dorsal pad and tubercles of fathead minnows (Pimephales promelas). Can J Zool. 1974 Aug;52(8):1031–1038. doi: 10.1139/z74-137. [DOI] [PubMed] [Google Scholar]
  30. Statham C. N., Croft W. A., Lech J. J. Uptake, distribution, and effects of carbon tetrachloride in rainbow trout (Salmo gairdneri). Toxicol Appl Pharmacol. 1978 Jul;45(1):131–140. doi: 10.1016/0041-008x(78)90034-0. [DOI] [PubMed] [Google Scholar]
  31. Sterio D. C. The unbiased estimation of number and sizes of arbitrary particles using the disector. J Microsc. 1984 May;134(Pt 2):127–136. doi: 10.1111/j.1365-2818.1984.tb02501.x. [DOI] [PubMed] [Google Scholar]
  32. Stäubli W., Hess R., Weibel E. R. Correlated morphometric and biochemical studies on the liver cell. II. Effects of phenobarbital on rat hepatocytes. J Cell Biol. 1969 Jul;42(1):92–112. doi: 10.1083/jcb.42.1.92. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Weibel E. R. Morphometric estimation of pulmonary diffusion capacity. I. Model and method. Respir Physiol. 1970;11(1):54–75. doi: 10.1016/0034-5687(70)90102-7. [DOI] [PubMed] [Google Scholar]
  34. Zuchelkowski E. M., Lantz R. C., Hinton D. E. Effects of acid-stress on epidermal mucous cells of the brown bullhead Ictalurus nebulosus (LeSeur): a morphometric study. Anat Rec. 1981 May;200(1):33–39. doi: 10.1002/ar.1092000104. [DOI] [PubMed] [Google Scholar]

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