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. 1987 Oct;74:49–53. doi: 10.1289/ehp.877449

Biomarkers in the detection of human heritable and germinal mutagenesis.

M L Mendelsohn 1
PMCID: PMC1474491  PMID: 3319555

Abstract

An important potential use of biomarkers in human toxicology is the detection of induced mutational events in offspring and germ cells of exposed individuals. The importance, of course, is in risk estimation and the identification and prevention of exposure conditions that are harmful to the human genome. The challenge is to discover methods of sufficient power to find the rare, random, mutational events and to discriminate such events from other sources of molecular variation. Finding mutations is essentially a search for disorder. Normal biomarkers are inherently unsuitable in a positive search for disorder; instead one must either use abnormal markers or be prepared to search negatively, i.e., to look for and somehow validate the rare absence of a normal marker. In spite of these difficulties, there is progress to report and hope of future success in this field.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Delehanty J., White R. L., Mendelsohn M. L. International Commission for Protection Against Environmental Mutagens and Carcinogens. ICPEMC Meeting Report No. 2. Approaches to determining mutation rates in human DNA. Mutat Res. 1986 May;167(3):215–232. doi: 10.1016/0165-1110(86)90031-x. [DOI] [PubMed] [Google Scholar]
  2. Jensen R. H., Vanderlaan M., Grabske R. J., Branscomb E. W., Bigbee W. L., Stanker L. H. Monoclonal antibodies specific for sickle cell hemoglobin. Hemoglobin. 1985;9(4):349–362. doi: 10.3109/03630268508997010. [DOI] [PubMed] [Google Scholar]
  3. Langlois R. G., Bigbee W. L., Jensen R. H. Measurements of the frequency of human erythrocytes with gene expression loss phenotypes at the glycophorin A locus. Hum Genet. 1986 Dec;74(4):353–362. doi: 10.1007/BF00280485. [DOI] [PubMed] [Google Scholar]
  4. Langlois R. G., Bigbee W. L., Kyoizumi S., Nakamura N., Bean M. A., Akiyama M., Jensen R. H. Evidence for increased somatic cell mutations at the glycophorin A locus in atomic bomb survivors. Science. 1987 Apr 24;236(4800):445–448. doi: 10.1126/science.3563520. [DOI] [PubMed] [Google Scholar]
  5. Myers R. M., Larin Z., Maniatis T. Detection of single base substitutions by ribonuclease cleavage at mismatches in RNA:DNA duplexes. Science. 1985 Dec 13;230(4731):1242–1246. doi: 10.1126/science.4071043. [DOI] [PubMed] [Google Scholar]
  6. O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
  7. Pinkel D., Straume T., Gray J. W. Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization. Proc Natl Acad Sci U S A. 1986 May;83(9):2934–2938. doi: 10.1073/pnas.83.9.2934. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Schull W. J., Otake M., Neel J. V. Genetic effects of the atomic bombs: a reappraisal. Science. 1981 Sep 11;213(4513):1220–1227. doi: 10.1126/science.7268429. [DOI] [PubMed] [Google Scholar]
  9. Schwartz D. C., Cantor C. R. Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis. Cell. 1984 May;37(1):67–75. doi: 10.1016/0092-8674(84)90301-5. [DOI] [PubMed] [Google Scholar]
  10. Skolnick M. M., Neel J. V. An algorithm for comparing two-dimensional electrophoretic gels, with particular reference to the study of mutation. Adv Hum Genet. 1986;15:55–160. doi: 10.1007/978-1-4615-8356-1_2. [DOI] [PubMed] [Google Scholar]

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