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. 1987 Aug;73:235–246. doi: 10.1289/ehp.8773235

Chemical and toxicological characterization of residential oil burner emissions: II. Mutagenic, tumorigenic, and potential teratogenic activity.

A G Braun 1, W F Busby Jr 1, H L Liber 1, W G Thilly 1
PMCID: PMC1474572  PMID: 3665866

Abstract

Extracts of effluents from a modern residential oil burner have been evaluated in several toxicological assay systems. Bacterial mutagens were detected in extracts from both the particulate and vapor phase emissions. Effluents from continuous operation were an order of magnitude less mutagenic than those from cyclic (5 min on, 10 min off) operations. No difference in the yield of bacterial mutagens per gram of fuel burned was found between cyclic operation under low and moderate sooting conditions. On the basis of elution behavior from alumina it appeared that the bacterial mutagens collected from high sooting effluents were more polar than those from low sooting effluent. An extract that was mutagenic in bacteria did not induce a significant increase in mutation frequency to human lymphoblasts. No evidence of tumorigenicity was observed in a limited number of newborn mice after IP injection of effluent extract when compared to historical control data. Putative nonmutagenic teratogens were detected in effluent using an attachment inhibition assay. The level of these agents was reduced in effluents from continuous oil burner operation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Braun A. G., Buckner C. A., Emerson D. J., Nichinson B. B. Quantitative correspondence between the in vivo and in vitro activity of teratogenic agents. Proc Natl Acad Sci U S A. 1982 Mar;79(6):2056–2060. doi: 10.1073/pnas.79.6.2056. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Braun A. G., Emerson D. J., Nichinson B. B. Teratogenic drugs inhibit tumour cell attachment to lectin-coated surfaces. Nature. 1979 Nov 29;282(5738):507–509. doi: 10.1038/282507a0. [DOI] [PubMed] [Google Scholar]
  3. Braun A. G., Nichinson B. B., Horowicz P. B. Inhibition of tumor cell attachment to concanavalin A-coated surfaces as an assay for teratogenic agents: approaches to validation. Teratog Carcinog Mutagen. 1982;2(3-4):343–354. doi: 10.1002/1520-6866(1990)2:3/4<343::aid-tcm1770020316>3.0.co;2-s. [DOI] [PubMed] [Google Scholar]
  4. Busby W. F., Jr, Goldman M. E., Newberne P. M., Wogan G. N. Tumorigenicity of fluoranthene in a newborn mouse lung adenoma bioassay. Carcinogenesis. 1984 Oct;5(10):1311–1316. doi: 10.1093/carcin/5.10.1311. [DOI] [PubMed] [Google Scholar]
  5. Crespi C. L., Thilly W. G. Assay for gene mutation in a human lymphoblast line, AHH-1, competent for xenobiotic metabolism. Mutat Res. 1984 Sep;128(2):221–230. doi: 10.1016/0027-5107(84)90110-6. [DOI] [PubMed] [Google Scholar]
  6. Furth E. E., Thilly W. G., Penman B. W., Liber H. L., Rand W. M. Quantitative assay for mutation in diploid human lymphoblasts using microtiter plates. Anal Biochem. 1981 Jan 1;110(1):1–8. doi: 10.1016/0003-2697(81)90103-2. [DOI] [PubMed] [Google Scholar]
  7. Lafleur A. L., Braun A. G., Monchamp P. A., Plummer E. F. Preserving toxicologic activity during chromatographic fractionation of bioactive complex mixtures. Anal Chem. 1986 Mar;58(3):568–572. doi: 10.1021/ac00294a017. [DOI] [PubMed] [Google Scholar]
  8. Leary J. A., Biemann K., Lafleur A. L., Kruzel E. L., Prado G. P., Longwell J. P., Peters W. A. Chemical and toxicological characterization of residential oil burner emissions: I. Yields and chemical characterization of extractables from combustion of No. 2 fuel oil at different Bacharach Smoke Numbers and firing cycles. Environ Health Perspect. 1987 Aug;73:223–234. doi: 10.1289/ehp.8773223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Nesnow S., Evans C., Stead A., Creason J., Slaga T. J., Triplett L. L. Skin carcinogenesis studies of emission extracts. Dev Toxicol Environ Sci. 1982;10:295–320. [PubMed] [Google Scholar]
  10. Peto R., Pike M. C., Day N. E., Gray R. G., Lee P. N., Parish S., Peto J., Richards S., Wahrendorf J. Guidelines for simple, sensitive significance tests for carcinogenic effects in long-term animal experiments. IARC Monogr Eval Carcinog Risk Chem Hum Suppl. 1980;(2 Suppl):311–426. [PubMed] [Google Scholar]
  11. Skopek T. R., Liber H. L., Kaden D. A., Thilly W. G. Relative sensitivities of forward and reverse mutation assays in Salmonella typhimurium. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4465–4469. doi: 10.1073/pnas.75.9.4465. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Skopek T. R., Liber H. L., Krolewski J. J., Thilly W. G. Quantitative forward mutation assay in Salmonella typhimurium using 8-azaguanine resistance as a genetic marker. Proc Natl Acad Sci U S A. 1978 Jan;75(1):410–414. doi: 10.1073/pnas.75.1.410. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Thilly W. G., Longwell J., Andon B. M. General approach to the biological analysis of complex mixtures. Environ Health Perspect. 1983 Feb;48:129–136. doi: 10.1289/ehp.8348129. [DOI] [PMC free article] [PubMed] [Google Scholar]

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