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. 1986 Mar;65:237–242. doi: 10.1289/ehp.65-1474698

Age-dependent response of the rat testes to di(2-ethylhexyl) phthalate.

P Sjöberg, N G Lindqvist, L Plöen
PMCID: PMC1474698  PMID: 3709447

Abstract

Spermatogenesis starts soon after birth in the Sprague-Dawley rat but is not fully established until about 56 days of age. When high oral doses of the plasticizer di(2-ethylhexyl) phthalate (DEHP) were given to rats of different ages, testicular damage was observed in immature but not in mature animals. Plasma concentration and urinary excretion data suggested that the gastrointestinal absorption of the DEHP-derived metabolite mono(2-ethylhexyl) phthalate was higher in young animals. Young rats were not more susceptible than older for repeated intravenous infusions of DEHP. It is suggested that the age-related difference observed in testicular response after oral administration of DEHP may be due to pharmacokinetic rather than tissue sensitivity differences. It is concluded that in assessing risks of testicular injuries in children exposed to DEHP, additional studies are required using species in which testicular development is more similar to that of humans.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Corpéchot C., Baulieu E. E., Robel P. Testosterone, dihydrotestosterone and androstanediols in plasma, testes and prostates of rats during development. Acta Endocrinol (Copenh) 1981 Jan;96(1):127–135. doi: 10.1530/acta.0.0960127. [DOI] [PubMed] [Google Scholar]
  2. Creasy D. M., Foster J. R., Foster P. M. The morphological development of di-N-pentyl phthalate induced testicular atrophy in the rat. J Pathol. 1983 Mar;139(3):309–321. doi: 10.1002/path.1711390307. [DOI] [PubMed] [Google Scholar]
  3. Ekwall H., Jansson A., Sjöberg P., Plöen L. Differentiation of the rat testis between 20 and 120 days of age. Arch Androl. 1984;13(1):27–36. doi: 10.3109/01485018408987498. [DOI] [PubMed] [Google Scholar]
  4. Foster P. M., Foster J. R., Cook M. W., Thomas L. V., Gangolli S. D. Changes in ultrastructure and cytochemical localization of zinc in rat testis following the administration of di-n-pentyl phthalate. Toxicol Appl Pharmacol. 1982 Mar 30;63(1):120–132. doi: 10.1016/0041-008x(82)90031-x. [DOI] [PubMed] [Google Scholar]
  5. Gangolli S. D. Testicular effects of phthalate esters. Environ Health Perspect. 1982 Nov;45:77–84. doi: 10.1289/ehp.824577. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gray T. J., Beamand J. A. Effect of some phthalate esters and other testicular toxins on primary cultures of testicular cells. Food Chem Toxicol. 1984 Feb;22(2):123–131. doi: 10.1016/0278-6915(84)90092-9. [DOI] [PubMed] [Google Scholar]
  7. Gray T. J., Butterworth K. R. Testicular atrophy produced by phthalate esters. Arch Toxicol Suppl. 1980;4:452–455. doi: 10.1007/978-3-642-67729-8_106. [DOI] [PubMed] [Google Scholar]
  8. Hagenäs L., Plöen L., Ekwall H., Osman D. I., Ritzén E. M. Differentiation of the rat seminiferous tubules between 13 and 19 days of age. Int J Androl. 1981 Apr;4(2):257–264. doi: 10.1111/j.1365-2605.1981.tb00709.x. [DOI] [PubMed] [Google Scholar]
  9. Huhtaniemi I. T., Nozu K., Warren D. W., Dufau M. L., Catt K. J. Acquisition of regulatory mechanisms for gonadotropin receptors and steroidogenesis in the maturing rat testis. Endocrinology. 1982 Nov;111(5):1711–1720. doi: 10.1210/endo-111-5-1711. [DOI] [PubMed] [Google Scholar]
  10. Jaeger R. J., Rubin R. J. Migration of a phthalate ester plasticizer from polyvinyl chloride blood bags into stored human blood and its localization in human tissues. N Engl J Med. 1972 Nov 30;287(22):1114–1118. doi: 10.1056/NEJM197211302872203. [DOI] [PubMed] [Google Scholar]
  11. Knorr D. W., Vanha-Perittula T., Lipsett M. B. Structure and function of rat testis through pubescence. Endocrinology. 1970 Jun;86(6):1298–1304. doi: 10.1210/endo-86-6-1298. [DOI] [PubMed] [Google Scholar]
  12. Lawrence W. H., Tuell S. F. Phthalate esters: the question of safety--an update. Clin Toxicol. 1979;15(4):447–466. doi: 10.3109/15563657908989899. [DOI] [PubMed] [Google Scholar]
  13. Mayer F. L., Stalling D. L., Johnson J. L. Phthalate esters as environmental contaminants. Nature. 1972 Aug 18;238(5364):411–413. doi: 10.1038/238411a0. [DOI] [PubMed] [Google Scholar]
  14. Murray H. E., Ray L. E., Giam C. S. Phthalic acid esters, total DDTs, and polychlorinated biphenyls in marine samples from Galveston Bay, Texas. Bull Environ Contam Toxicol. 1981 Jun;26(6):769–774. doi: 10.1007/BF01622169. [DOI] [PubMed] [Google Scholar]
  15. Ogner G., Schnitzer M. Humic substances: fulvic acid-dialkyl phthalate complexes and their role in pollution. Science. 1970 Oct 16;170(3955):317–318. doi: 10.1126/science.170.3955.317. [DOI] [PubMed] [Google Scholar]
  16. Roosen-Runge E. C., Leik J. Gonocyte degeneration in the postnatal male rat. Am J Anat. 1968 Mar;122(2):275–299. doi: 10.1002/aja.1001220208. [DOI] [PubMed] [Google Scholar]
  17. Seth P. K. Hepatic effects of phthalate esters. Environ Health Perspect. 1982 Nov;45:27–34. doi: 10.1289/ehp.824527. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sjöberg P. O., Bondesson U. G., Sedin E. G., Gustafsson J. P. Exposure of newborn infants to plasticizers. Plasma levels of di-(2-ethylhexyl) phthalate and mono-(2-ethylhexyl) phthalate during exchange transfusion. Transfusion. 1985 Sep-Oct;25(5):424–428. doi: 10.1046/j.1537-2995.1985.25586020115.x. [DOI] [PubMed] [Google Scholar]
  19. Sjöberg P., Bondesson U., Kjellen L., Lindquist N. G., Montin G., Plöen L. Kinetics of di-(2-ethylhexyl) phthalate in immature and mature rats and effect on testis. Acta Pharmacol Toxicol (Copenh) 1985 Jan;56(1):30–37. doi: 10.1111/j.1600-0773.1985.tb01249.x. [DOI] [PubMed] [Google Scholar]
  20. Vitale R., Fawcett D. W., Dym M. The normal development of the blood-testis barrier and the effects of clomiphene and estrogen treatment. Anat Rec. 1973 Jul;176(3):331–344. doi: 10.1002/ar.1091760309. [DOI] [PubMed] [Google Scholar]
  21. de Jong F. H., Sharpe R. M. The onset and establishment of spermatogenesis in rats in relation to gonadotrophin and testosterone levels. J Endocrinol. 1977 Nov;75(2):197–207. doi: 10.1677/joe.0.0750197. [DOI] [PubMed] [Google Scholar]

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