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. 1974 Aug;8:157–164. doi: 10.1289/ehp.748157

Biochemical Parameters of Guinea Pig Perilymph Sampled According to Scala and Following Sound Presentation

Leon L Gershbein, Dennis T Manshio, Phil S Shurrager
PMCID: PMC1474943  PMID: 4470918

Abstract

Guinea pigs were exposed to sound varying from 2 to 8 kHz in frequency and 80-100 dB (SPL) in intensity for periods of 1 hr. The biochemical parameters, glucose, sodium, total protein, and the glycolytic enzymes, aldolase, phosphohexose isomerase, and total LDH as well as isozymes of the latter were ascertained for blood serum, perilymph, and, in some instances, cerebrospinal fluid. The three enzymes occurred at lower levels in perilymph as compared to blood serum. Except for a small difference in serum total protein, sound presentation incurred no significant effect on any of the above parameters. Definite differences in several metabolites were discerned for perilymph sampled according to scala and which were independent of the respective acoustical treatments. Thus, as compared to the scale tympani, the scala vestibuli perilymph displayed a higher glucose content and a diminished total LDH level and of the latter isozymes, LDH1 ranged lower and LDH2, higher. As further evidence pointing to cerebrospinal fluid as the possible origin of perilymph, similarities in glucose contents and LDH isozyme patterns were noted for both fluids.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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