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. 1998 May 1;26(9):2075–2081. doi: 10.1093/nar/26.9.2075

Transcription activation by Escherichia coli FNR protein: similarities to, and differences from, the CRP paradigm.

B Li 1, H Wing 1, D Lee 1, H C Wu 1, S Busby 1
PMCID: PMC147511  PMID: 9547262

Abstract

During transcription activation at FNR-dependent promoters where the DNA site for FNR overlaps the -35 element, a surface-exposed activating region in the upstream subunit of the FNR dimer interacts with the C-terminal domain of the RNA polymerase alpha subunit. Starting with a cloned fnr gene encoding a defective FNR derivative carrying substitutions in this activating region, we screened a library of random mutations to identify substitutions that restored FNR activity. Activity can be restored by substitutions at residues T118, E47 and K60. The locations of these residues identify three separate surface-exposed regions of FNR that can play a role in transcription activation. These three regions appear to be analogues of Activating Region 1, Activating Region 2 and Activating Region 3 of the cyclic AMP receptor protein, CRP: our results underscore the similarities between FNR and CRP.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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