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. 1998 Jun 1;26(11):2829–2830. doi: 10.1093/nar/26.11.2829

An alternative PCR assay for quantifying mitochondrial DNA in crude preparations.

T C Koekemoer 1, T G Downing 1, W Oelofsen 1
PMCID: PMC147619  PMID: 9592176

Abstract

A method is described for the quantification of mitochondrial DNA present in crude biological preparations. A known copy number of a standard is amplified in the presence of inactivated target DNA so as to determine the overall efficiency of the PCR process in a particular sample. In this way any inhibitory and/or stimulatory substances present in sample preparations can be taken into account. To reduce tube-to-tube variations product DNA quantification is limited to small cycle numbers. Using this method quantitations of DNA amounts in different crude preparations can be compared.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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