Abstract
The viability of an attenuated live virus rabies vaccine in a bait targeted to red foxes was examined under various operational conditions in a series of experiments in Ontario. The virus was relatively stable over a 28-day period in the field, losing a mean 0.5, s = 0.2 log10 of virus titer. The micro-environment into which the bait was placed (open cultivated field, grassy meadow, wooded grove, sun or shade) did not make an appreciable difference in the viability of the virus. There was no significant difference (P < or = 0.05) between mean ambient temperatures and the temperature of fluids in blister packs of baits placed in sun or shade. Sixty-three percent of foxes fed baits exposed to sun and shade conditions for 21 days (titer 10(6.2) tissue culture infective doses per 1 mL) developed rabies virus-neutralizing antibodies. Storage of vaccine baits at -30 degrees C prior to bait distribution was important in maintaining virus viability.
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Selected References
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- Abelseth M. K. An Attenuated Rabies Vaccine for Domestic Animals Produced in Tissue Culture. Can Vet J. 1964 Nov;5(11):279–286. [PMC free article] [PubMed] [Google Scholar]
- Abreo C. B. A rapid quantitative microtest for live, attenuated rabies vaccine. J Biol Stand. 1985 Jul;13(3):255–260. doi: 10.1016/s0092-1157(85)80009-3. [DOI] [PubMed] [Google Scholar]
- Bachmann P., Bramwell R. N., Fraser S. J., Gilmore D. A., Johnston D. H., Lawson K. F., MacInnes C. D., Matejka F. O., Miles H. E., Pedde M. A. Wild carnivore acceptance of baits for delivery of liquid rabies vaccine. J Wildl Dis. 1990 Oct;26(4):486–501. doi: 10.7589/0090-3558-26.4.486. [DOI] [PubMed] [Google Scholar]
- Barton L. D., Campbell J. B. Measurement of rabies-specific antibodies in carnivores by an enzyme-linked immunosorbent assay. J Wildl Dis. 1988 Apr;24(2):246–258. doi: 10.7589/0090-3558-24.2.246. [DOI] [PubMed] [Google Scholar]
- Johnston D. H., Voigt D. R., MacInnes C. D., Bachmann P., Lawson K. F., Rupprecht C. E. An aerial baiting system for the distribution of attenuated or recombinant rabies vaccines for foxes, raccoons, and skunks. Rev Infect Dis. 1988 Nov-Dec;10 (Suppl 4):S660–S664. doi: 10.1093/clinids/10.supplement_4.s660. [DOI] [PubMed] [Google Scholar]
- Lawson K. F., Chiu H., Crosgrey S. J., Matson M., Casey G. A., Campbell J. B. Duration of immunity in foxes vaccinated orally with ERA vaccine in a bait. Can J Vet Res. 1997 Jan;61(1):39–42. [PMC free article] [PubMed] [Google Scholar]
- Lawson K. F., Chiu H., Matson M., Bachmann P., Campbell J. B. Studies on efficacy and stability of a vaccine bait containing ERA strain of rabies virus propagated in a BHK-21 cell line. Can J Vet Res. 1992 Apr;56(2):135–141. [PMC free article] [PubMed] [Google Scholar]
- Lawson K. F., Hertler R., Charlton K. M., Campbell J. B., Rhodes A. J. Safety and immunogenicity of ERA strain of rabies virus propagated in a BHK-21 cell line. Can J Vet Res. 1989 Oct;53(4):438–444. [PMC free article] [PubMed] [Google Scholar]
- MacInnes C. D., Tinline R. R., Voigt D. R., Broekhoven L. H., Rosatte R. R. Planning for rabies control in Ontario. Rev Infect Dis. 1988 Nov-Dec;10 (Suppl 4):S665–S669. doi: 10.1093/clinids/10.supplement_4.s665. [DOI] [PubMed] [Google Scholar]
- Zalan E., Wilson C., Pukitis D. A microtest for the quantitation of rabies virus neutralizing antibodies. J Biol Stand. 1979 Jul;7(3):213–220. doi: 10.1016/s0092-1157(79)80024-4. [DOI] [PubMed] [Google Scholar]