Abstract
A study was conducted in 2 feedlots in southern Alberta to identify environmental sources and management factors associated with the prevalence and transmission of Escherichia coli O157:H7. Escherichia coli O157:H7 was isolated in preslaughter pens of cattle from feces (0.8%), feedbunks (1.7%), water troughs (12%), and incoming water supplies (4.5%), but not from fresh total mixed rations. Fresh total mixed rations did not support the growth of E. coli O157:H7 and E. coli from bovine feces following experimental inoculation. Within a feedlot, the feces, water troughs, and feedbunks shared a few indistinguishable subtypes of E. coli O157:H7. A few subtypes were repeatedly isolated in the same feedlot, and the 2 feedlots shared a few indistinguishable subtypes. The prevalence of E. coli O157:H7 in water troughs of preslaughter cattle in 1 feedlot was associated with season, maximum climatic temperatures the week before sampling; total precipitation the week before sampling, and coliform and E. coli counts in the water trough.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- Chapman P. A., Siddons C. A., Gerdan Malo A. T., Harkin M. A. A 1-year study of Escherichia coli O157 in cattle, sheep, pigs and poultry. Epidemiol Infect. 1997 Oct;119(2):245–250. doi: 10.1017/s0950268897007826. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gannon V. P., D'Souza S., Graham T., King R. K., Rahn K., Read S. Use of the flagellar H7 gene as a target in multiplex PCR assays and improved specificity in identification of enterohemorrhagic Escherichia coli strains. J Clin Microbiol. 1997 Mar;35(3):656–662. doi: 10.1128/jcm.35.3.656-662.1997. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hancock D. D., Besser T. E., Rice D. H., Herriott D. E., Tarr P. I. A longitudinal study of Escherichia coli O157 in fourteen cattle herds. Epidemiol Infect. 1997 Apr;118(2):193–195. doi: 10.1017/s0950268896007212. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rice D. H., McMenamin K. M., Pritchett L. C., Hancock D. D., Besser T. E. Genetic subtyping of Escherichia coli O157 isolates from 41 Pacific Northwest USA cattle farms. Epidemiol Infect. 1999 Jun;122(3):479–484. doi: 10.1017/s0950268899002496. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sanderson M. W., Gay J. M., Hancock D. D., Gay C. C., Fox L. K., Besser T. E. Sensitivity of bacteriologic culture for detection of Escherichia coli O157:H7 in bovine feces. J Clin Microbiol. 1995 Oct;33(10):2616–2619. doi: 10.1128/jcm.33.10.2616-2619.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Van Donkersgoed J., Graham T., Gannon V. The prevalence of verotoxins, Escherichia coli O157:H7, and Salmonella in the feces and rumen of cattle at processing. Can Vet J. 1999 May;40(5):332–338. [PMC free article] [PubMed] [Google Scholar]