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. 2001 Nov;42(11):857–860.

The immunohistochemical detection of Mycoplasma bovis and bovine viral diarrhea virus in tissues of feedlot cattle with chronic, unresponsive respiratory disease and/or arthritis.

D M Haines 1, K M Martin 1, E G Clark 1, G K Jim 1, E D Janzen 1
PMCID: PMC1476660  PMID: 11708203

Abstract

The purpose of this study was to determine the frequency of selected pathogens in the tissues of a group of feedlot cattle with chronic disease (most often respiratory disease and/or arthritis). Samples of lung and joint tissues from 49 feedlot animals that had failed to respond to antibiotic therapy were tested by immunohistochemical staining for the antigens of Mycoplasma bovis, Haemophilus somnus, Pasteurella (Mannheimia) hemolytica, and bovine viral diarrhea virus (BVDV). Mycoplasma bovis was demonstrated in over 80% of cases, including in 45% of joints and 71% of lungs tested. Mycoplasma bovis was the only bacterial pathogen identified in the joints. Haemophilus somnus and Pasteurella (Mannheimia) haemolytica were found in 14% and 23% of cases, respectively, and were confined to the lungs in all instances. Infection with BVDV was demonstrated in over 40% of cases. Mycoplasma bovis and BVDV were the most common pathogens persisting in the tissues of these animals that had failed to respond to antibiotic therapy.

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Selected References

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  1. Adegboye D. S., Halbur P. G., Nutsch R. G., Kadlec R. G., Rosenbusch R. F. Mycoplasma bovis-associated pneumonia and arthritis complicated with pyogranulomatous tenosynovitis in calves. J Am Vet Med Assoc. 1996 Aug 1;209(3):647–649. [PubMed] [Google Scholar]
  2. Booker C. W., Guichon P. T., Jim G. K., Schunicht O. C., Harland R. J., Morley P. S. Seroepidemiology of undifferentiated fever in feedlot calves in western Canada. Can Vet J. 1999 Jan;40(1):40–48. [PMC free article] [PubMed] [Google Scholar]
  3. Ellis J. A., West K. H., Cortese V. S., Myers S. L., Carman S., Martin K. M., Haines D. M. Lesions and distribution of viral antigen following an experimental infection of young seronegative calves with virulent bovine virus diarrhea virus-type II. Can J Vet Res. 1998 Jul;62(3):161–169. [PMC free article] [PubMed] [Google Scholar]
  4. Haines D. M., Chelack B. J. Technical considerations for developing enzyme immunohistochemical staining procedures on formalin-fixed paraffin-embedded tissues for diagnostic pathology. J Vet Diagn Invest. 1991 Jan;3(1):101–112. doi: 10.1177/104063879100300128. [DOI] [PubMed] [Google Scholar]
  5. Martin S. W., Bateman K. G., Shewen P. E., Rosendal S., Bohac J. G., Thorburn M. A group level analysis of the associations between antibodies to seven putative pathogens and respiratory disease and weight gain in Ontario feedlot calves. Can J Vet Res. 1990 Jun;54(3):337–342. [PMC free article] [PubMed] [Google Scholar]
  6. Njaa B. L., Clark E. G., Janzen E., Ellis J. A., Haines D. M. Diagnosis of persistent bovine viral diarrhea virus infection by immunohistochemical staining of formalin-fixed skin biopsy specimens. J Vet Diagn Invest. 2000 Sep;12(5):393–399. doi: 10.1177/104063870001200501. [DOI] [PubMed] [Google Scholar]
  7. Potgieter L. N. Immunology of bovine viral diarrhea virus. Vet Clin North Am Food Anim Pract. 1995 Nov;11(3):501–520. doi: 10.1016/s0749-0720(15)30464-3. [DOI] [PubMed] [Google Scholar]
  8. Radostits O. M., Janzen E., Doige C., Jim K. Alberta. Mycoplasma arthritis in feedlot cattle. Can Vet J. 1988 Jun;29(6):531–532. [PMC free article] [PubMed] [Google Scholar]
  9. Rosendal S., Martin S. W. The association between serological evidence of mycoplasma infection and respiratory disease in feedlot calves. Can J Vet Res. 1986 Apr;50(2):179–183. [PMC free article] [PubMed] [Google Scholar]
  10. Taylor L. F., Van Donkersgoed J., Dubovi E. J., Harland R. J., van den Hurk J. V., Ribble C. S., Janzen E. D. The prevalence of bovine viral diarrhea virus infection in a population of feedlot calves in western Canada. Can J Vet Res. 1995 Apr;59(2):87–93. [PMC free article] [PubMed] [Google Scholar]
  11. Van Donkersgoed J., Janzen E. D., Harland R. J. Epidemiological features of calf mortality due to hemophilosis in a large feedlot. Can Vet J. 1990 Dec;31(12):821–825. [PMC free article] [PubMed] [Google Scholar]
  12. Van Donkersgoed J., Janzen E. D., Potter A. A., Harland R. J. The occurrence of Haemophilus somnus in feedlot calves and its control by postarrival prophylactic mass medication. Can Vet J. 1994 Sep;35(9):573–580. [PMC free article] [PubMed] [Google Scholar]

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