Skip to main content

Some NLM-NCBI services and products are experiencing heavy traffic, which may affect performance and availability. We apologize for the inconvenience and appreciate your patience. For assistance, please contact our Help Desk at info@ncbi.nlm.nih.gov.

Nucleic Acids Research logoLink to Nucleic Acids Research
. 1998 Jul 15;26(14):3447–3448. doi: 10.1093/nar/26.14.3447

Efficient modification of a human chromosome by telomere-directed truncation in high homologous recombination-proficient chicken DT40 cells.

Y Kuroiwa 1, T Shinohara 1, T Notsu 1, K Tomizuka 1, H Yoshida 1, S Takeda 1, M Oshimura 1, I Ishida 1
PMCID: PMC147703  PMID: 9649633

Abstract

Truncation of human chromosomes at desired sites by homologous recombination techniques enables functional and structural analyses of human chromosomes and development of human artificial chromosomes. However, this targeted truncation has been inefficient. We describe here an efficient method for targeted truncation in the chicken DT40 cells with a high homologous recombination rate. The human chromosome 22 was transferred into DT40 cells, where human telomeric repeat (TTAGGG)n was targeted to the LIF locus on the chromosome. Molecular and cytogenetic analyses showed that the predicted truncation at the LIF locus occurred in all of the targeted clones.

Full Text

The Full Text of this article is available as a PDF (50.5 KB).


Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES