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. 1998 Aug 15;26(16):3862–3864. doi: 10.1093/nar/26.16.3862

In situ detection of protein-DNA interactions in filamentous fungi by in vivo footprinting.

M F Wolschek 1, F Narendja 1, J Karlseder 1, C P Kubicek 1, C Scazzocchio 1, J Strauss 1
PMCID: PMC147760  PMID: 9685506

Abstract

The method described here allows the detection of protein-DNA interactions in vivo in filamentous fungi. We outline culture conditions and conditions of in vivo methylation that permit uniform modification of all cells in an apically growing, non-uniform organism, and subsequent visualization of protected areas by ligation-mediated PCR.

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Selected References

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