Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1998 Oct 15;26(20):4785–4786. doi: 10.1093/nar/26.20.4785

A convenient method of aligning large DNA molecules on bare mica surfaces for atomic force microscopy.

J Li 1, C Bai 1, C Wang 1, C Zhu 1, Z Lin 1, Q Li 1, E Cao 1
PMCID: PMC147909  PMID: 9753751

Abstract

Large DNA molecules remain difficult to be imaged by atomic force microscopy (AFM) because of the tendency of aggregation. A method is described to align long DNA fibers in a single direction on unmodified mica to facilitate AFM studies. The clear background, minimal overstretching, high reproducibility and convenience of this aligning procedure make it useful for physical mapping of genome regions and the studies of DNA-protein complexes.

Full Text

The Full Text of this article is available as a PDF (84.0 KB).

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES