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. 1958 Mar;13(1):54–61. doi: 10.1111/j.1476-5381.1958.tb00190.x

Continuous recording of acid gastric secretion in the rat

M N Ghosh, H O Schild
PMCID: PMC1481706  PMID: 13523135

Abstract

A method is described for the continuous recording of acid gastric secretion in the rat. The stomach of the rat anaesthetized with urethane is perfused with a dilute sodium hydroxide solution by way of the oesophagus and the pH of the fluid emerging from a cannula in the pylorus is registered graphically. In passing through the stomach, the perfusate collects sufficient buffer to act as an approximately linear buffer system over the relevant range, so that the change in pH becomes a measure of acid secretion. The preparation is suitable for the bioassay of secretory stimulants. Ten or more drug doses can be administered in succession in one preparation, so that each animal serves as a self-contained assay unit. Intravenous doses of histamine, methacholine, carbachol and acetylcholine produce a graded and reversible stimulation of acid secretion. The secretory effect of histamine is markedly and specifically potentiated by antihistaminases.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ARUNLAKSHANA O., MONGAR J. L., SCHILD H. O. Potentiation of pharmacological effects of histamine by histaminase inhibitors. J Physiol. 1954 Jan;123(1):32–54. doi: 10.1113/jphysiol.1954.sp005032. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Babkin B. P. THE ABNORMAL FUNCTIONING OF THE GASTRIC SECRETORY MECHANISM AS A POSSIBLE FACTOR IN THE PATHOGENESIS OF PEPTIC ULCER. Can Med Assoc J. 1938 May;38(5):421–429. [PMC free article] [PubMed] [Google Scholar]
  3. GHOSH M. N., SCHILD H. O. A method for the continuous recording of acid gastric secretion in the rat. J Physiol. 1955 May 27;128(2):35–6P. [PubMed] [Google Scholar]
  4. IVY A. C., IVY E. K., KARVINEN E., LIN T. M. Effect of histaminase inhibitors on gastric secretion. Am J Physiol. 1956 Aug;186(2):239–244. doi: 10.1152/ajplegacy.1956.186.2.239. [DOI] [PubMed] [Google Scholar]
  5. PEVSNER L., GROSSMAN M. I. The mechanism of vagal stimulation of gastric acid secretion. Gastroenterology. 1955 Apr;28(4):493-9; discussion, 531-5. [PubMed] [Google Scholar]
  6. ROVELSTAD R. A., OWEN C. A., Jr, MAGATH T. B. Factors influencing the continuous recording of in situ pH of gastric and duodenal contents. Gastroenterology. 1952 Apr;20(4):609–624. [PubMed] [Google Scholar]
  7. SCHULER W. Zur Hemmung der Diaminooxydase (Histaminase). Experientia. 1952 Jun 15;8(6):230–232. doi: 10.1007/BF02170726. [DOI] [PubMed] [Google Scholar]
  8. SIRCUS W. The effect of B1-pyrimidine on gastric secretion. Q J Exp Physiol Cogn Med Sci. 1953;38(1):25–33. doi: 10.1113/expphysiol.1953.sp001004. [DOI] [PubMed] [Google Scholar]
  9. WATON N. G. Studies on mammalian histidine decarboxylase. Br J Pharmacol Chemother. 1956 Jun;11(2):119–127. doi: 10.1111/j.1476-5381.1956.tb01039.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. WESTLING H. Potentiation of histamine effects by histaminase inhibitors in the unanaesthetized guinea-pig. Acta Physiol Scand. 1956 Dec 31;38(2):91–120. doi: 10.1111/j.1748-1716.1957.tb01375.x. [DOI] [PubMed] [Google Scholar]

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