Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1999 Jan 15;27(2):426–428. doi: 10.1093/nar/27.2.426

A vector based on the SV40 origin of replication and chromosomal S/MARs replicates episomally in CHO cells.

C Piechaczek 1, C Fetzer 1, A Baiker 1, J Bode 1, H J Lipps 1
PMCID: PMC148196  PMID: 9862961

Abstract

We have developed an episomal replicating expression vector in which the SV40 gene coding for the large T-antigen was replaced by chromosomal scaffold/matrix attached regions. Southern analysis as well as vector rescue experiments in CHO cells and in Escherichia coli demonstrate that the vector replicates episomally in CHO cells. It occurs in a very low copy number in the cells and is stably maintained over more than 100 generations without selection pressure.

Full Text

The Full Text of this article is available as a PDF (116.4 KB).

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES