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. 1962 Dec;19(3):427–441. doi: 10.1111/j.1476-5381.1962.tb01447.x

An action of 5-hydroxytryptamine on adrenaline receptors

I R Innes
PMCID: PMC1482220  PMID: 13956570

Abstract

Contractions of isolated strips of cat spleen due to 5-hydroxytryptamine, adrenaline, histamine and acetylcholine were antagonized by phenoxybenzamine. Responses to both 5-hydroxytryptamine and adrenaline were not blocked in strips which were protected by a high concentration of either 5-hydroxytryptamine or adrenaline throughout exposure to phenoxybenzamine. The contraction due to a large dose of 5-hydroxytryptamine lasted less than 1 hr even when the drug was still present. Strips thus desensitized to 5-hydroxytryptamine responded normally to acetylcholine and histamine but did not respond to adrenaline. The actions of 5-hydroxytryptamine and adrenaline were blocked by 2-bromolysergic acid diethylamide or by dihydroergotamine. These results indicated that 5-hydroxytryptamine and adrenaline act on the same receptors. Cocaine potentiated the action of adrenaline but inhibited the action of 5-hydroxytryptamine. The sensitivity to 5-hydroxytryptamine of spleen strips from cats treated 24 hr earlier with reserpine was only one-fiftieth of that of normal strips. Cocaine potentiated the action of 5-hydroxytryptamine on strips from reserpine-treated cats. A high concentration of 5-hydroxytryptamine in spleen strips from reserpine-treated cats and in cocaine-treated strips prevented phenoxybenzamine from blocking the actions of adrenaline. The effects of tyramine on spleen strips almost exactly paralleled the effects of 5-hydroxytryptamine. Strips showing tachyphylaxis to tyramine did not respond to 5-hydroxytryptamine. It is concluded that 5-hydroxytryptamine has a dual action, viz., a major action due to release of stored noradrenaline and a minor direct action of adrenaline receptors.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BURN J. H., RAND M. J. The action of sympathomimetic amines in animals treated with reserpine. J Physiol. 1958 Dec 4;144(2):314–336. doi: 10.1113/jphysiol.1958.sp006104. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. ERSPAMER V. Pharmacological studies on enteramine (5-hydroxytryptamine) IX. Influence of sympathomimetic and sympatholytic drugs on the physiological and pharmacological actions of enteramine. Arch Int Pharmacodyn Ther. 1953 Apr;93(3-4):293–316. [PubMed] [Google Scholar]
  3. FURCHGOTT R. F. Dibenamine blockade in strips of rabbit aorta and its use in differentiating receptors. J Pharmacol Exp Ther. 1954 Jul;111(3):265–284. [PubMed] [Google Scholar]
  4. GADDUM J. H., HAMEED K. A. Drugs which antagonize 5-hydroxytryptamine. Br J Pharmacol Chemother. 1954 Jun;9(2):240–248. doi: 10.1111/j.1476-5381.1954.tb00848.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. GADDUM J. H., HAMEED K. A., HATHWAY D. E., STEPHENS F. F. Quantitative studies of antagonists for 5-hydroxytryptamine. Q J Exp Physiol Cogn Med Sci. 1955 Jan;40(1):49–74. doi: 10.1113/expphysiol.1955.sp001097. [DOI] [PubMed] [Google Scholar]
  6. GADDUM J. H., PICARELLI Z. P. Two kinds of tryptamine receptor. Br J Pharmacol Chemother. 1957 Sep;12(3):323–328. doi: 10.1111/j.1476-5381.1957.tb00142.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. GADDUM J. H. Tryptamine receptors. J Physiol. 1953 Feb 27;119(2-3):363–368. doi: 10.1113/jphysiol.1953.sp004851. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. GINZEL K. H., KOTTEGODA S. R. A study of the vascular actions of 5-hydroxytryptamine, tryptamine, adrenaline and noradrenaline. Q J Exp Physiol Cogn Med Sci. 1953;38(4):225–231. doi: 10.1113/expphysiol.1953.sp001033. [DOI] [PubMed] [Google Scholar]
  9. HERTTING G., AXELROD J., PATRICK R. W. Actions of cocaine and tyramine on the uptake and release of H3-norepinephrine in the heart. Biochem Pharmacol. 1961 Sep;8:246–248. doi: 10.1016/0006-2952(61)90009-0. [DOI] [PubMed] [Google Scholar]
  10. LOCKETT M. F., EAKINS K. E. Chromatographic studies of the effect of intravenous injections of tyramine on the concentrations of adrenaline and noradrenaline in plasma. J Pharm Pharmacol. 1960 Sep;12:513–517. doi: 10.1111/j.2042-7158.1960.tb12702.x. [DOI] [PubMed] [Google Scholar]
  11. MEIER R., TRIPOD J., WIRZ E. Classification d'une série d'antagonistes de la sérotonine et analyse de ses points d'attaque vasculaires périphériques. Arch Int Pharmacodyn Ther. 1957 Jan 1;109(1-2):55–77. [PubMed] [Google Scholar]
  12. MUSCHOLL E. Effect of cocaine and related drugs on the uptake of noradrenaline by heart and spleen. Br J Pharmacol Chemother. 1961 Jun;16:352–359. doi: 10.1111/j.1476-5381.1961.tb01095.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. WHITBY L. G., HERTTING G., AXELROD J. Effect of cocaine on the disposition of noradrenaline labelled with tritium. Nature. 1960 Aug 13;187:604–605. doi: 10.1038/187604a0. [DOI] [PubMed] [Google Scholar]

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