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. 1971 Mar;11(3):295–301. doi: 10.1016/S0006-3495(71)86215-X

Deoxyribonucleic Acid Synthesis in Ultraviolet-Light-Irradiated Chinese Hamster Cells

Raymond E Meyn, Ronald M Humphrey
PMCID: PMC1483991  PMID: 5103274

Abstract

Two mammalian cell lines, Chinese hamster ovary (CHO) which can recover colony-forming ability between fractionated doses of ultraviolet light (UV), and Chinese hamster B-14FAF28 which cannot recover, were tested for the ability to bypass UV-induced photoproducts in DNA during postirradiation DNA synthesis. The molecular weight distributions of newly synthesized DNA in UV-irradiated populations of both cell lines showed evidence for photoproduct bypass. Hence, the bypass mechanism does not correlate with recovery after UV.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOYCE R. P., HOWARD-FLANDERS P. RELEASE OF ULTRAVIOLET LIGHT-INDUCED THYMINE DIMERS FROM DNA IN E. COLI K-12. Proc Natl Acad Sci U S A. 1964 Feb;51:293–300. doi: 10.1073/pnas.51.2.293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cleaver J. E. Repair replication in Chinese hamster cells after damage from ultraviolet light. Photochem Photobiol. 1970 Jul;12(1):17–28. doi: 10.1111/j.1751-1097.1970.tb06033.x. [DOI] [PubMed] [Google Scholar]
  3. Cleaver J. E., Thomas G. H. Single strand interruptions in DNA and the effects of caffeine in Chinese hamster cells irradiated with ultraviolet light. Biochem Biophys Res Commun. 1969 Jul 23;36(2):203–208. doi: 10.1016/0006-291x(69)90315-5. [DOI] [PubMed] [Google Scholar]
  4. Cleaver J. R., Painter R. B. Evidence for repair replication of HeLa cell DNA damaged by ultraviolet light. Biochim Biophys Acta. 1968 Jul 23;161(2):552–554. doi: 10.1016/0005-2787(68)90131-7. [DOI] [PubMed] [Google Scholar]
  5. Domon M., Rauth A. M. Ultraviolet-light irradiation of mouse L cells: effects on cells in the DNA synthesis phase. Radiat Res. 1969 Nov;40(2):414–429. [PubMed] [Google Scholar]
  6. HUMPHREY R. M., DEWEY W. C., CORK A. Relative ultraviolet sensitivity of different phases in the cell cycle of Chinese hamster cells grown in vitro. Radiat Res. 1963 Jun;19:247–260. [PubMed] [Google Scholar]
  7. Humphrey R. M., Sedita B. A., Meyn R. E. Recovery of Chinese hamster cells from ultra-violet irradiation damage. Int J Radiat Biol Relat Stud Phys Chem Med. 1970;18(1):61–69. doi: 10.1080/09553007014550821. [DOI] [PubMed] [Google Scholar]
  8. Humphrey R. M., Steward D. L., Sedita B. A. DNA-strand breaks and rejoining following exposure of synchronized Chinese hamster cells to ionizing radiations. Mutat Res. 1968 Nov-Dec;6(3):459–465. doi: 10.1016/0027-5107(68)90063-8. [DOI] [PubMed] [Google Scholar]
  9. Klímek M. Thymine dimerization in L-strain mammalian cells after irradiation with ultraviolet light and the search for repair mechanisms. Photochem Photobiol. 1966 Aug;5(8):603–607. doi: 10.1111/j.1751-1097.1966.tb05806.x. [DOI] [PubMed] [Google Scholar]
  10. Noll H. Characterization of macromolecules by constant velocity sedimentation. Nature. 1967 Jul 22;215(5099):360–363. doi: 10.1038/215360a0. [DOI] [PubMed] [Google Scholar]
  11. PETTIJOHN D., HANAWALT P. EVIDENCE FOR REPAIR-REPLICATION OF ULTRAVIOLET DAMAGED DNA IN BACTERIA. J Mol Biol. 1964 Aug;9:395–410. doi: 10.1016/s0022-2836(64)80216-3. [DOI] [PubMed] [Google Scholar]
  12. Painter R. B., Cleaver J. E. Repair replication, unscheduled DNA synthesis, and the repair of mammalian DNA. Radiat Res. 1969 Mar;37(3):451–466. [PubMed] [Google Scholar]
  13. Rauth A. M. Evidence for dark-reactivation of ultraviolet light damage in mouse L cells. Radiat Res. 1967 May;31(1):121–138. [PubMed] [Google Scholar]
  14. Regan J. D., Trosko J. E., Carrier W. L. Evidence for excision of ultraviolet-induced pyrimidine dimers from the DNA of human cells in vitro. Biophys J. 1968 Mar;8(3):319–325. doi: 10.1016/S0006-3495(68)86490-2. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rupp W. D., Howard-Flanders P. Discontinuities in the DNA synthesized in an excision-defective strain of Escherichia coli following ultraviolet irradiation. J Mol Biol. 1968 Jan 28;31(2):291–304. doi: 10.1016/0022-2836(68)90445-2. [DOI] [PubMed] [Google Scholar]
  16. SETLOW R. B., CARRIER W. L. THE DISAPPEARANCE OF THYMINE DIMERS FROM DNA: AN ERROR-CORRECTING MECHANISM. Proc Natl Acad Sci U S A. 1964 Feb;51:226–231. doi: 10.1073/pnas.51.2.226. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Setlow R. B., Regan J. D., German J., Carrier W. L. Evidence that xeroderma pigmentosum cells do not perform the first step in the repair of ultraviolet damage to their DNA. Proc Natl Acad Sci U S A. 1969 Nov;64(3):1035–1041. doi: 10.1073/pnas.64.3.1035. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. TROSKO J. E., CHU E. H., CARRIER W. L. THE INDUCTION OF THYMINE DIMERS IN ULTRAVIOLET-IRRADIATED MAMMALIAN CELLS. Radiat Res. 1965 Apr;24:667–672. [PubMed] [Google Scholar]

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