Skip to main content
NCBI home page
Biophysical Journal logoLink to Biophysical Journal
. 1971 Feb;11(2):189–203. doi: 10.1016/S0006-3495(71)86207-0

The Influence of Two Naturally Occurring Factors, from the Leaves of Ricinus Communis, on the Fluorescence Induction of Higher Plant Chloroplasts and Intact Algae

Marcia Brody
PMCID: PMC1484014  PMID: 5547552

Abstract

Changes in fluorescence induction, brought about by incubation of chloroplasts (Zea mays) in an aqueous extract of Ricinus leaf, have been divided, on the basis of speed of manifestation, into two categories: “fast” changes and “slow” changes (i.e. those observed after 5 min and 1½ hr of incubation, respectively). The former, which include a large increase in the magnitude of the fast component of variable fluorescence and a retardation of decay from maximum to minimum levels of fluorescence, have been ascribed to inhibition of electron transport at a site beyond that of 3-(p-chlorophenyl)-1,1-dimethylurea (CMU)—i.e., towards system I; these changes result from the action of a fraction of the extract consisting of molecules of small size. The latter changes, which include a marked attenuation of the variable part of fluorescence induction, have been associated with system II and may arise from inhibition of electron flow between water and Q or from decrease in number of functional reaction centers; these changes result from the activity of a proteinaceous fraction of the extract, that simultaneously converts the low temperature steady-state emission spectrum of the chloroplasts into a one-banded one, with maximum at 698 nm.

Full text

PDF
189

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BROYDE S. B., BRODY S. S. F698: A PIGMENT FOUND IN PREPARATIONS OF CHLOROPHYLL. Biochem Biophys Res Commun. 1965 May 3;19:444–451. doi: 10.1016/0006-291x(65)90144-0. [DOI] [PubMed] [Google Scholar]
  3. Brody M., Linschitz H. Fluorescence of Photosynthetic Organisms at Room and Liquid Nitrogen Temperatures. Science. 1961 Mar 10;133(3454):705–706. doi: 10.1126/science.133.3454.705. [DOI] [PubMed] [Google Scholar]
  4. Brody M., Nathanson B., Cohen W. S. Enchancement of emission from chloroplasts at 698 nm by naturally-occurring factor. Biochim Biophys Acta. 1969 Feb 25;172(2):340–342. doi: 10.1016/0005-2728(69)90079-6. [DOI] [PubMed] [Google Scholar]
  5. Cheniae G. M., Martin I. F. Site of manganese function in photosynthesis. Biochim Biophys Acta. 1968 May 28;153(4):819–837. doi: 10.1016/0005-2728(68)90009-1. [DOI] [PubMed] [Google Scholar]
  6. Cohen W. S., Nathanson B., White J. E., Brody M. Fatty acids as model systems for the action of Ricinus leaf extract on higher plant chloroplasts and algae. Arch Biochem Biophys. 1969 Dec;135(1):21–27. doi: 10.1016/0003-9861(69)90511-6. [DOI] [PubMed] [Google Scholar]
  7. Delosme R. Etude de l'induction de fluorescence des algues vertes et des chloroplastes au début d'une illumination intense. Biochim Biophys Acta. 1967 Jul 5;143(1):108–128. doi: 10.1016/0005-2728(67)90115-6. [DOI] [PubMed] [Google Scholar]
  8. Forbush B., Kok B. Reaction between primary and secondary electron acceptors of photosystem II of photosynthesis. Biochim Biophys Acta. 1968 Aug 20;162(2):243–253. doi: 10.1016/0005-2728(68)90106-0. [DOI] [PubMed] [Google Scholar]
  9. GOEDHEER J. C. FLUORESCENCE BANDS AND CHLOROPHYLL A FORMS. Biochim Biophys Acta. 1964 Sep 25;88:304–317. doi: 10.1016/0926-6577(64)90186-x. [DOI] [PubMed] [Google Scholar]
  10. JAGENDORF A. T., AVRON M. Cofactors and rates of photosynthetic phosphorylation by spinach chloroplasts. J Biol Chem. 1958 Mar;231(1):277–290. [PubMed] [Google Scholar]
  11. JOLIOT P., LAVOREL J. LES R'EACTIONS PRIMAIRES DE LA PHOTOSYNTH'ESE. Bull Soc Chim Biol (Paris) 1964;46:1607–1626. [PubMed] [Google Scholar]
  12. Joliot P. Cinétiques des réactions liées a l'émission d'oxygène photosynthétique. Biochim Biophys Acta. 1965 May 25;102(1):116–134. [PubMed] [Google Scholar]
  13. Joliot P., Joliot A., Kok B. Analysis of the interactions between the two photosystems in isolated chloroplasts. Biochim Biophys Acta. 1968 Apr 2;153(3):635–652. doi: 10.1016/0005-2728(68)90191-6. [DOI] [PubMed] [Google Scholar]
  14. LAVOREL J. H'ETEROG'EN'EIT'E DE LA CHLOROPHYLLE IN VIVO. II. POLARISATION ET SPECTRES D'ACTION DE FLUORESCENCE. Biochim Biophys Acta. 1964 Jul 29;88:20–36. [PubMed] [Google Scholar]
  15. LAVOREL J., LEMASSON C. [New demonstration of the Mehler reaction in Chlorella]. Biochim Biophys Acta. 1961 May 27;49:574–577. doi: 10.1016/0006-3002(61)90255-4. [DOI] [PubMed] [Google Scholar]
  16. Lavorel J. Induction of Fluorescence in Quinone Poisoned Chlorella Cells. Plant Physiol. 1959 May;34(3):204–209. doi: 10.1104/pp.34.3.204. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. McCarty R. E., Jagendorf A. T. Chloroplast damage due to enzymatic hydrolysis of endogenous lipids. Plant Physiol. 1965 Jul;40(4):725–735. doi: 10.1104/pp.40.4.725. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Murata N., Nishimura M., Takamiya A. Fluorescene of chlorophyll in photosynthetic systems. II. Induction of fluorescence in isolated spinach chloroplasts. Biochim Biophys Acta. 1966 May 12;120(1):23–33. doi: 10.1016/0926-6585(66)90273-1. [DOI] [PubMed] [Google Scholar]

Articles from Biophysical Journal are provided here courtesy of The Biophysical Society

RESOURCES