Skip to main content
Biophysical Journal logoLink to Biophysical Journal
. 1972 Feb;12(2):175–190. doi: 10.1016/S0006-3495(72)86078-8

Direct Determination of the Structure of Barium Stearate Multilayers by X-Ray Diffraction

W Lesslauer, J K Blasie
PMCID: PMC1484062  PMID: 5057749

Abstract

Diffraction of X-rays is recorded from barium stearate multilayer systems with from 2 to 60 double layers or unit cells. The generalized Patterson function P′(x) is calculated by an integral Fourier transform of observed intensity data from a specimen containing only two unit cells. The Patterson function P0(x) of a single unit cell is determined from P′(x) and the electron density distribution of a bimolecular leaflet is obtained by a deconvolution procedure of P0(x) after Hosemann and Bagchi. The electron density distribution is also calculated independently by a conventional Fourier synthesis with an experimentally established set of phases. The results of the two methods are consistent and fit a physical model of the bimolecular leaflet. A direct analysis, therefore, can be performed if diffraction is observed from multilayer systems with a small number of unit cells.

Full text

PDF
175

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blaurock A. E., Worthington C. R. Treatment of low angle x-ray data from planar and concentric multilayered structures. Biophys J. 1966 May;6(3):305–312. doi: 10.1016/S0006-3495(66)86658-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. ELLIOTT G. F., WORTHINGTON C. R. A SMALL-ANGLE OPTICALLY FOCUSING X-RAY DIFFRACTION CAMERA IN BIOLOGICAL RESEARCH. I. J Ultrastruct Res. 1963 Aug;49:166–170. doi: 10.1016/s0022-5320(63)80044-1. [DOI] [PubMed] [Google Scholar]
  3. FINEAN J. B. X-ray diffraction studies on the polymorphism of phospholipids. Biochim Biophys Acta. 1953 Mar;10(3):371–384. doi: 10.1016/0006-3002(53)90268-6. [DOI] [PubMed] [Google Scholar]
  4. Gras W. J., Worthington C. R. X-ray analysis of retinal photoreceptors. Proc Natl Acad Sci U S A. 1969 Jun;63(2):233–238. doi: 10.1073/pnas.63.2.233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Levine Y. K., Bailey A. I., Wilkins M. H. Multilayers of phospholipid bimolecular leaflets. Nature. 1968 Nov 9;220(5167):577–578. doi: 10.1038/220577a0. [DOI] [PubMed] [Google Scholar]
  6. Steim J. M., Tourtellotte M. E., Reinert J. C., McElhaney R. N., Rader R. L. Calorimetric evidence for the liquid-crystalline state of lipids in a biomembrane. Proc Natl Acad Sci U S A. 1969 May;63(1):104–109. doi: 10.1073/pnas.63.1.104. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biophysical Journal are provided here courtesy of The Biophysical Society

RESOURCES