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Nucleic Acids Research logoLink to Nucleic Acids Research
. 1999 May 1;27(9):1966–1969. doi: 10.1093/nar/27.9.1966

The chicken HPRT gene: a counter selectable marker for the DT40 cell line.

T Fukagawa 1, N Hayward 1, J Yang 1, C Azzalin 1, D Griffin 1, A F Stewart 1, W Brown 1
PMCID: PMC148408  PMID: 10198428

Abstract

We describe the cloning, characterisation and chromosomal mapping of the chicken hprt gene together with the construction of two counter selectable hprt-/- DT40 derived cell lines. One of these cell lines contains a stably integrated gene encoding a conditionally active cre recombinase and thus allows efficient manipulation of targeted loci by site-specific recombination. These cell lines will enhance the utility of the hyper-recombinogenic DT40 cell line as a system for the genetic analysis of cell autonomous functions in vertebrates and as a tool for mammalian chromosome engineering.


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