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. 1971 Dec;11(12):981–994. doi: 10.1016/S0006-3495(71)86272-0

The Rate Constants of Valinomycin-Mediated Ion Transport through Thin Lipid Membranes

G Stark, B Ketterer, R Benz, P Läuger
PMCID: PMC1484112  PMID: 4332419

Abstract

Electrical relaxation experiments have been performed with phosphatidylinositol bilayer membranes in the presence of the ion carrier valinomycin. After a sudden change of the voltage a relaxation of the membrane current with a time constant of about 20 μsec is observed. Together with previous stationary conductance data, the relaxation amplitude and the relaxation time are used to evaluate the rate constants of valinomycin-mediated potassium transport across the lipid membrane. It is found that the rate constants of translocation of the free carrier S and the carrier-ion complex MS+ are nearly equal (2·104 sec-1) and are of the same order as the dissociation rate constant of MS+ in the membrane-solution interface (5·104 sec-1). The equilibrium constant of the heterogeneous association reaction M+ (solution) + S (membrane) → MS+ (membrane) is found to be ∼ 1 M-1, about 106 times smaller than the association constant in ethanolic solution.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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