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. 1972 Nov;12(11):1405–1414. doi: 10.1016/S0006-3495(72)86171-X

An X-Ray Diffraction Study of Contracting Molluscan Smooth Muscle

B M Millman, G F Elliott
PMCID: PMC1484200  PMID: 4642219

Abstract

The living anterior byssus retractor muscle of Mytilus (ABRM), a smooth, “catch” muscle, has been studied by X-ray diffraction while relaxed and while tonically contracted. X-ray reflections were observed from the actin and paramyosin filaments and from the α-helical substructure of the paramyosin filaments. No differences in spacings or relative intensities were observed when the relaxed and contracting muscle patterns were compared. This result is consistent with a sliding filament mechanism involving an interaction between actin and paramyosin filaments.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BEAR R. S., SELBY C. C. The structure of paramyosin fibrils according to x-ray diffraction. J Biophys Biochem Cytol. 1956 Jan 25;2(1):55–69. doi: 10.1083/jcb.2.1.55. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. COHEN C., HOLMES K. C. X-ray diffraction evidence for alpha-helical coiled-coils in native muscle. J Mol Biol. 1963 May;6:423–432. doi: 10.1016/s0022-2836(63)80053-4. [DOI] [PubMed] [Google Scholar]
  3. ELLIOTT G. F., WORTHINGTON C. R. A SMALL-ANGLE OPTICALLY FOCUSING X-RAY DIFFRACTION CAMERA IN BIOLOGICAL RESEARCH. I. J Ultrastruct Res. 1963 Aug;49:166–170. doi: 10.1016/s0022-5320(63)80044-1. [DOI] [PubMed] [Google Scholar]
  4. Elliott A., Lowy J., Parry D. A., Vibert P. J. Puzzle of the coiled coils in the alpha-protein paramyosin. Nature. 1968 May 18;218(5142):656–659. doi: 10.1038/218656a0. [DOI] [PubMed] [Google Scholar]
  5. Elliott G. F., Lowy J., Millman B. M. X-ray diffraction from living striated muscle during contraction. Nature. 1965 Jun 26;206(991):1357–1358. doi: 10.1038/2061357a0. [DOI] [PubMed] [Google Scholar]
  6. Huxley H. E., Brown W., Holmes K. C. Constancy of axial spacings in frog sartorius muscle during contraction. Nature. 1965 Jun 26;206(991):1358–1358. doi: 10.1038/2061358a0. [DOI] [PubMed] [Google Scholar]
  7. JOHNSON W. H., KAHN J. S., SZENTGYORGYI A. G. Paramyosin and contraction of catch muscles. Science. 1959 Jul 17;130(3368):160–161. doi: 10.1126/science.130.3368.160. [DOI] [PubMed] [Google Scholar]
  8. LOWY J., HANSON J. Ultrastructure of invertebrate smooth muscles. Physiol Rev Suppl. 1962 Jul;5:34–47. [PubMed] [Google Scholar]
  9. LOWY J., MILLMAN B. M., HANSON J. STRUCTURE AND FUNCTION IN SMOOTH TONIC MUSCLES OF LAMELLIBRANCH MOLLUSCS. Proc R Soc Lond B Biol Sci. 1964 Oct 27;160:525–536. doi: 10.1098/rspb.1964.0068. [DOI] [PubMed] [Google Scholar]
  10. Lowy J., Vibert P. J. Structure and organization of actin in a molluscan smooth muscle. Nature. 1967 Sep 16;215(5107):1254–1255. doi: 10.1038/2151254a0. [DOI] [PubMed] [Google Scholar]
  11. Mandelkern L., Posner A. S., Diorio A. F., Laki K. MECHANISM OF CONTRACTION IN THE MUSCLE FIBER-ATP SYSTEM. Proc Natl Acad Sci U S A. 1959 Jun;45(6):814–819. doi: 10.1073/pnas.45.6.814. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Millman B. M., Elliott G. F. X-ray diffraction from contracting molluscan muscle. Nature. 1965 May 22;206(4986):824–825. doi: 10.1038/206824a0. [DOI] [PubMed] [Google Scholar]
  13. PAUTARD F. G. Proton transfer and supercontraction in actomyosin. Nature. 1959 May 16;183(4672):1391–1392. doi: 10.1038/1831391b0. [DOI] [PubMed] [Google Scholar]
  14. SELBY C. C., BEAR R. S. The structure of actin-rich filaments of muscles according to x-ray diffraction. J Biophys Biochem Cytol. 1956 Jan 25;2(1):71–85. doi: 10.1083/jcb.2.1.71. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Szent-Györgyi A. G., Cohen C., Kendrick-Jones J. Paramyosin and the filaments of molluscan "catch" muscles. II. Native filaments: isolation and characterization. J Mol Biol. 1971 Mar 14;56(2):239–258. doi: 10.1016/0022-2836(71)90462-1. [DOI] [PubMed] [Google Scholar]

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