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. 1973 Feb;13(2):167–178. doi: 10.1016/S0006-3495(73)85977-6

Caloric Catastrophe

Lawrence Minkoff, Raymond Damadian
PMCID: PMC1484216  PMID: 4573826

Abstract

Six solutes known to be actively transported by bacteria were studied with the cell in a “minimum energy” state to determine if sufficient energy were available from cellular stores of ATP to supply the energy necessary to run postulated membrane-situated “pumps.” Steady-state cellular concentrations of potassium, calcium, magnesium, leucine, glycine, and α-methyl glucoside were determined together with tracer fluxes, oxygen consumption, ATP turnover, and the P:O ratio. From these measurements, it was calculated that the energy supply, 4.20 cal/340 min-g dry wt, fell far short of the energy necessary (28.28 cal/340 min-g dry wt), by classical membrane theory, to operate “pumps.”

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOYER P. D., ROBBINS E. A. Determination of the equilibrium of the hexokinase reaction and the free energy of hydrolysis of adenosine triphosphate. J Biol Chem. 1957 Jan;224(1):121–135. [PubMed] [Google Scholar]
  2. CRANE R. K., LIPMANN F. The effect of arsenate on aerobic phosphorylation. J Biol Chem. 1953 Mar;201(1):235–243. [PubMed] [Google Scholar]
  3. DAWES E. A., RIBBONS D. W. The endogenous metabolism of microorganisms. Annu Rev Microbiol. 1962;16:241–264. doi: 10.1146/annurev.mi.16.100162.001325. [DOI] [PubMed] [Google Scholar]
  4. Damadian R. Biological ion exchanger resins. 3. Molecular interpretation of cellular ion exchange. Biophys J. 1971 Sep;11(9):773–785. doi: 10.1016/s0006-3495(71)86253-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Damadian R. Biological ion exchanger resins. I. Quantitative electrostatic correspondence of fixed charge and mobile counter ion. Biophys J. 1971 Sep;11(9):739–760. doi: 10.1016/S0006-3495(71)86251-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Damadian R., Goldsmith M., Zaner K. S. Biological ion exchanger resins. II. QUERP water and ion exchange selectivity. Biophys J. 1971 Sep;11(9):761–772. doi: 10.1016/S0006-3495(71)86252-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Damadian R. Ion metabolism in a potassium accumulation mutant of Escherichia coli B. I. Potassium metabolism. J Bacteriol. 1968 Jan;95(1):113–122. doi: 10.1128/jb.95.1.113-122.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dreizen P., Hartshorne D. J., Stracher A. The subunit structure of myosin. I. Polydispersity in 5 M guanidine. J Biol Chem. 1966 Jan 25;241(2):443–448. [PubMed] [Google Scholar]
  9. Green K. Ion transport in isolated cornea of the rabbit. Am J Physiol. 1965 Dec;209(6):1311–1316. doi: 10.1152/ajplegacy.1965.209.6.1311. [DOI] [PubMed] [Google Scholar]
  10. Kaback H. R., Stadtman E. R. Proline uptake by an isolated cytoplasmic membrane preparation of Escherichia coli. Proc Natl Acad Sci U S A. 1966 Apr;55(4):920–927. doi: 10.1073/pnas.55.4.920. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Levine M., Oxender D. L., Stein W. D. The substrate-facilitated transport of the glucose carrier across the human erythrocyte membrane. Biochim Biophys Acta. 1965 Sep 27;109(1):151–163. doi: 10.1016/0926-6585(65)90099-3. [DOI] [PubMed] [Google Scholar]
  12. Pardee A. B., Prestidge L. S., Whipple M. B., Dreyfuss J. A binding site for sulfate and its relation to sulfate transport into Salmonella typhimurium. J Biol Chem. 1966 Sep 10;241(17):3962–3969. [PubMed] [Google Scholar]
  13. SCHULTZ S. G., SOLOMON A. K. Cation transport in Escherichia coli. I. Intracellular Na and K concentrations and net cation movement. J Gen Physiol. 1961 Nov;45:355–369. doi: 10.1085/jgp.45.2.355. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. SCHULTZ S. G., WILSON N. L., EPSTEIN W. Cation transport in Escherichia coli. II. Intracellular chloride concentration. J Gen Physiol. 1962 Sep;46:159–166. doi: 10.1085/jgp.46.1.159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. SKOU J. C. ENZYMATIC BASIS FOR ACTIVE TRANSPORT OF NA+ AND K+ ACROSS CELL MEMBRANE. Physiol Rev. 1965 Jul;45:596–617. doi: 10.1152/physrev.1965.45.3.596. [DOI] [PubMed] [Google Scholar]
  16. SOLOMON A. K. Ion transport in single cell populations. Biophys J. 1962 Mar;2(2 Pt 2):79–95. doi: 10.1016/s0006-3495(62)86949-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Silver S. Active transport of magnesium in escherichia coli. Proc Natl Acad Sci U S A. 1969 Mar;62(3):764–771. doi: 10.1073/pnas.62.3.764. [DOI] [PMC free article] [PubMed] [Google Scholar]

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