Skip to main content
PMC home page
Biophysical Journal logoLink to Biophysical Journal
. 1972 Jul;12(7):922–929. doi: 10.1016/S0006-3495(72)86134-4

A Spectroscopic Analysis of a High Fluorescent Mutant of Chlamydomonas Reinhardi

B L Epel, W L Butler
PMCID: PMC1484260  PMID: 5037344

Abstract

Chloroplast fragments of a high fluorescent mutant of Chlamydomonas reinhardi, hfd 91, were compared against those of Acl+, a low chlorophyll variant of the wild type. The chloroplast fragments of the mutant which have a high invariant fluorescence yield lacked photochemical activities associated with photosystem II (PSII) but retained normal photosystem I (PSI) activities. The mutant fragments also lacked the low temperature (-196°C) light-induced absorbance changes due to the photoreduction of C-550 and the photooxidation of cytochrome (cyt) b-559 which are PSII-mediated reactions. A fourth-derivative analysis of the absolute spectra of the chloroplast fragments at different stages of reduction (obtained with ferricyanide, ascorbate, and dithionite) showed both the oxidized and reduced forms of C-550 and the reduced forms of cyt c-553, b-559, and b-564 in wild-type fragments. The mutant fragments lacked C-550 and an ascorbate-reducible cyt b-559 but contained cyt c-553, a dithionite-reducible cyt b-559, and cyt b-564.

Full text

PDF
922

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bennoun P., Levine R. P. Detecting mutants that have impaired photosynthesis by their increased level of fluorescence. Plant Physiol. 1967 Sep;42(9):1284–1287. doi: 10.1104/pp.42.9.1284. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Boardman N. K., Anderson J. M. Fractionation of the photochemical systems of photosynthesis. II. Cytochrome and carotenoid contents of particles isolated from spinach chloroplasts. Biochim Biophys Acta. 1967 Jul 5;143(1):187–203. doi: 10.1016/0005-2728(67)90120-x. [DOI] [PubMed] [Google Scholar]
  4. Epel B. L., Levine R. P. Mutant strains of Chlamydomonas reinhardi with lesions on the oxidizing side of photosystem II. Biochim Biophys Acta. 1971 Jan 12;226(1):154–160. doi: 10.1016/0005-2728(71)90187-3. [DOI] [PubMed] [Google Scholar]
  5. Erixon K., Lozier R., Butler W. L. The redox state of cytochrome b 559 in spinach chloroplasts. Biochim Biophys Acta. 1972 May 25;267(2):375–382. doi: 10.1016/0005-2728(72)90124-7. [DOI] [PubMed] [Google Scholar]
  6. Gorman D. S., Levine R. P. Cytochrome f and plastocyanin: their sequence in the photosynthetic electron transport chain of Chlamydomonas reinhardi. Proc Natl Acad Sci U S A. 1965 Dec;54(6):1665–1669. doi: 10.1073/pnas.54.6.1665. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Knaff D. B., Arnon D. I. LIGHT-INDUCED OXIDATION OF A CHLOROPLAST B-TYPE CYTOCHROME AT -189 degrees C. Proc Natl Acad Sci U S A. 1969 Jul;63(3):956–962. doi: 10.1073/pnas.63.3.956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Knaff D. B., Arnon D. I. Spectral evidence for a new photoreactive component of the oxygen-evolving system in photosynthesis. Proc Natl Acad Sci U S A. 1969 Jul;63(3):963–969. doi: 10.1073/pnas.63.3.963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Levine R. P., Gorman D. S., Avron M., Butler W. L. Light-induced absorbance changes in wild-type and mutant strains of Chlamydomonas reinhardi. Brookhaven Symp Biol. 1966;19:143–148. [PubMed] [Google Scholar]
  10. Levine R. P., Gorman D. S. Photosynthetic electron transport chain of Chlamydomonas reinhardi. 3. Light-induced absorbance changes in chloroplast fragments of the wild type and mutant strains. Plant Physiol. 1966 Oct;41(8):1293–1300. doi: 10.1104/pp.41.8.1293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. NORRIS K. H., BUTLER W. L. Techniques for obtaining absorption spectra on intact biological samples. Ire Trans Biomed Electron. 1961 Jul;8:153–157. doi: 10.1109/tbmel.1961.4322890. [DOI] [PubMed] [Google Scholar]
  12. Yamashita T., Butler W. L. Photoreduction and photophosphorylation with tris-washed chloroplasts. Plant Physiol. 1968 Dec;43(12):1978–1986. doi: 10.1104/pp.43.12.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biophysical Journal are provided here courtesy of The Biophysical Society

RESOURCES