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. 1999 Sep 1;27(17):e15. doi: 10.1093/nar/27.17.e15

A novel quantitative multiplex NASBA method: application to measuring tissue factor and CD14 mRNA levels in human monocytes.

P B van Deursen 1, A W Gunther 1, C C van Riel 1, M M van der Eijnden 1, H L Vos 1, B van Gemen 1, D A van Strijp 1, N M Tackent 1, R M Bertina 1
PMCID: PMC148576  PMID: 10446258

Abstract

A new method to quantify two individual mRNAs in a single NASBA reaction is described. In this study, tissue factor and CD14 mRNAs were used as a model system. RNA ratios of -4 to +4 log units were determined with good precision (within 0.3 log) and accuracy (within 0.2 log). By measuring both mRNAs in human monocytes that were stimulated with LPS, the multiplex Q-NASBA proved to be a successful tool to monitor the expression levels of two individual mRNAs in a single-tube amplification system. The method has potential in all fields in which quantitative information is needed on two individual RNAs.

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