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. 1987 Feb;205(2):175–181. doi: 10.1097/00000658-198702000-00012

Hypothermia-induced reversible platelet dysfunction.

C R Valeri, H Feingold, G Cassidy, G Ragno, S Khuri, M D Altschule
PMCID: PMC1492823  PMID: 3813688

Abstract

Baboons that were subjected to systemic hypothermia at 32 C had an arm skin temperature of 27.3 C and bleeding time of 5.8 minutes. With local warming of the arm skin to 34 C, the bleeding time was 2.4 minutes. In normothermic baboons with arm skin temperature of 34.6 C, the bleeding time was 3.1 minutes. Local cooling of the arm skin to 27.6 C produced a bleeding time of 6.9 minutes. Increasing the skin temperature of the arm in hypothermic baboons to 38.9 C and in normothermic baboons to 40.1 C reduced bleeding times to 2.1 and 2.3 minutes, respectively. In both hypothermic and normothermic baboons there was a negative and significant correlation between the bleeding time and the arm skin temperature and the thromboxane B2 level in the shed blood obtained at the template bleeding time site. There was a significant positive correlation between the thromboxane B2 level in the shed blood and the arm skin temperature. Both in-vivo and in-vitro studies have shown that the production of thromboxane B2 by platelets is temperature-dependent, and that a cooling of skin temperature produces a reversible platelet dysfunction. Data also suggest that when a hypothermic patient bleeds without surgical cause, skin and wound temperature should be restored to normal before the administration of blood products that are not only expensive but may also transmit disease.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Costa J. L., Tanaka Y., Pettigrew K., Cushing R. J. Evaluation of the utility of air-dried whole mounts for quantitative electron microprobe studies of platelet dense bodies. J Histochem Cytochem. 1977 Sep;25(9):1079–1086. doi: 10.1177/25.9.333020. [DOI] [PubMed] [Google Scholar]
  2. Di Minno G., Martinez J., McKean M. L., De La Rosa J., Burke J. F., Murphy S. Platelet dysfunction in uremia. Multifaceted defect partially corrected by dialysis. Am J Med. 1985 Nov;79(5):552–559. doi: 10.1016/0002-9343(85)90051-8. [DOI] [PubMed] [Google Scholar]
  3. Feingold H. M., Pivacek L. E., Melaragno A. J., Valeri C. R. Coagulation assays and platelet aggregation patterns in human, baboon, and canine blood. Am J Vet Res. 1986 Oct;47(10):2197–2199. [PubMed] [Google Scholar]
  4. Harker L. A., Malpass T. W., Branson H. E., Hessel E. A., 2nd, Slichter S. J. Mechanism of abnormal bleeding in patients undergoing cardiopulmonary bypass: acquired transient platelet dysfunction associated with selective alpha-granule release. Blood. 1980 Nov;56(5):824–834. [PubMed] [Google Scholar]
  5. Harker L. A., Slichter S. J. The bleeding time as a screening test for evaluation of platelet function. N Engl J Med. 1972 Jul 27;287(4):155–159. doi: 10.1056/NEJM197207272870401. [DOI] [PubMed] [Google Scholar]
  6. Melaragno A. J., Abdu W., Katchis R., Doty A., Valeri C. R. Liquid and freeze preservation of baboon platelets. Cryobiology. 1981 Oct;18(5):445–452. doi: 10.1016/0011-2240(81)90202-9. [DOI] [PubMed] [Google Scholar]
  7. Mielke C. H., Jr, Kaneshiro M. M., Maher I. A., Weiner J. M., Rapaport S. I. The standardized normal Ivy bleeding time and its prolongation by aspirin. Blood. 1969 Aug;34(2):204–215. [PubMed] [Google Scholar]
  8. Moncada S., Vane J. R. Arachidonic acid metabolites and the interactions between platelets and blood-vessel walls. N Engl J Med. 1979 May 17;300(20):1142–1147. doi: 10.1056/NEJM197905173002006. [DOI] [PubMed] [Google Scholar]
  9. Quick A. J. Salicylates and bleeding: the aspirin tolerance test. Am J Med Sci. 1966 Sep;252(3):265–269. doi: 10.1097/00000441-196609000-00003. [DOI] [PubMed] [Google Scholar]
  10. Thompson C. B., Eaton K. A., Princiotta S. M., Rushin C. A., Valeri C. R. Size dependent platelet subpopulations: relationship of platelet volume to ultrastructure, enzymatic activity, and function. Br J Haematol. 1982 Mar;50(3):509–519. doi: 10.1111/j.1365-2141.1982.tb01947.x. [DOI] [PubMed] [Google Scholar]
  11. Thompson C. B., Love D. G., Quinn P. G., Valeri C. R. Platelet size does not correlate with platelet age. Blood. 1983 Aug;62(2):487–494. [PubMed] [Google Scholar]
  12. Valeri C. R. Circulation and hemostatic effectiveness of platelets stored at 4 C or 22 C: studies in aspirin-treated normal volunteers. Transfusion. 1976 Jan-Feb;16(1):20–23. doi: 10.1046/j.1537-2995.1976.16176130832.x. [DOI] [PubMed] [Google Scholar]
  13. Vecchione J. J., Melaragno A. J., Weiblen B. J., Halkett J. A., Callow A. D., Valeri C. R. Repeated intravenous administrations of human albumin and human fibrinogen in the baboon: survival measurements. J Med Primatol. 1982;11(2):91–105. doi: 10.1159/000460040. [DOI] [PubMed] [Google Scholar]

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