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. 1987 Nov;206(5):612–620. doi: 10.1097/00000658-198711000-00010

An in vitro study of the properties influencing Staphylococcus epidermidis adhesion to prosthetic vascular graft materials.

J M Harris 1, L F Martin 1
PMCID: PMC1493300  PMID: 2960278

Abstract

This study examines the influence of the properties of various vascular graft materials on the bacterial adherence process of two different strains of Staphylococcus epidermidis (mucous and normucous producing). Dacron grafts (both knitted and woven), Teflon grafts, and Dacron grafts coated with one and two layers of silicone were studied because these materials differ significantly in porosity, hydrophobicity, and surface charge (zeta potential). Graft segments were immersed in 3H-labeled bacteria solution for periods ranging from 5 to 180 minutes and liquid scintillation techniques were used to quantify bacterial adherence. The porous knitted Dacron material had a significantly higher rate of bacterial adherence than either the woven Dacron or Teflon (p less than 0.05). Silicone coating (either one or two layers) reduced adherence by a factor of four for the knitted Dacron (p less than 0.05) and by a factor of two for woven Dacron (p less than 0.05). The mucous producing strain of S. epidermidis displayed significantly better adherence to woven and knitted Dacron than the normucous producing strain, but only when 0.25% dextrose was added to the bacteria solution. These findings indicate that the highly porous knitted Dacron grafts have the highest propensity for bacterial adhesion. Graft materials with the most negative zeta potentials are more resistant to bacterial adherence. Silicone coating of Dacron material significantly changed adherence characteristics, suggesting that this may be a viable strategy for protecting implantable medical devices containing materials to which bacteria readily adhere.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abbott A., Rutter P. R., Berkeley R. C. The influence of ionic strength, pH and a protein layer on the interaction between Streptococcus mutans and glass surfaces. J Gen Microbiol. 1983 Feb;129(2):439–445. doi: 10.1099/00221287-129-2-439. [DOI] [PubMed] [Google Scholar]
  2. Absolom D. R., Lamberti F. V., Policova Z., Zingg W., van Oss C. J., Neumann A. W. Surface thermodynamics of bacterial adhesion. Appl Environ Microbiol. 1983 Jul;46(1):90–97. doi: 10.1128/aem.46.1.90-97.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Archer G. L. Antimicrobial susceptibility and selection of resistance among Staphylococcus epidermidis isolates recovered from patients with infections of indwelling foreign devices. Antimicrob Agents Chemother. 1978 Sep;14(3):353–359. doi: 10.1128/aac.14.3.353. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ashar B., Ward R. S., Jr, Turcotte L. R. Development of a silica-free silicone system for medical applications. J Biomed Mater Res. 1981 Sep;15(5):663–672. doi: 10.1002/jbm.820150505. [DOI] [PubMed] [Google Scholar]
  5. Bandyk D. F., Berni G. A., Thiele B. L., Towne J. B. Aortofemoral graft infection due to Staphylococcus epidermidis. Arch Surg. 1984 Jan;119(1):102–108. doi: 10.1001/archsurg.1984.01390130084015. [DOI] [PubMed] [Google Scholar]
  6. Brown G. L., Richardson J. D., Malangoni M. A., Tobin G. R., Ackerman D., Polk H. C., Jr Comparison of prosthetic materials for abdominal wall reconstruction in the presence of contamination and infection. Ann Surg. 1985 Jun;201(6):705–711. doi: 10.1097/00000658-198506000-00006. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Buckels J. A., Fielding J. W., Black J., Ashton F., Slaney G. Significance of positive bacterial cultures from aortic aneurysm contents. Br J Surg. 1985 Jun;72(6):440–442. doi: 10.1002/bjs.1800720610. [DOI] [PubMed] [Google Scholar]
  8. Bunt T. J. Synthetic vascular graft infections. I. Graft infections. Surgery. 1983 Jun;93(6):733–746. [PubMed] [Google Scholar]
  9. Christensen G. D., Simpson W. A., Bisno A. L., Beachey E. H. Adherence of slime-producing strains of Staphylococcus epidermidis to smooth surfaces. Infect Immun. 1982 Jul;37(1):318–326. doi: 10.1128/iai.37.1.318-326.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Christensen G. D., Simpson W. A., Bisno A. L., Beachey E. H. Experimental foreign body infections in mice challenged with slime-producing Staphylococcus epidermidis. Infect Immun. 1983 Apr;40(1):407–410. doi: 10.1128/iai.40.1.407-410.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Chu C. C., Williams D. F. Effects of physical configuration and chemical structure of suture materials on bacterial adhesion. A possible link to wound infection. Am J Surg. 1984 Feb;147(2):197–204. doi: 10.1016/0002-9610(84)90088-6. [DOI] [PubMed] [Google Scholar]
  12. Costerton J. W., Geesey G. G., Cheng K. J. How bacteria stick. Sci Am. 1978 Jan;238(1):86–95. doi: 10.1038/scientificamerican0178-86. [DOI] [PubMed] [Google Scholar]
  13. FOLKMAN J., LONG D. M. THE USE OF SILICONE RUBBER AS A CARRIER FOR PROLONGED DRUG THERAPY. J Surg Res. 1964 Mar;4:139–142. doi: 10.1016/s0022-4804(64)80040-8. [DOI] [PubMed] [Google Scholar]
  14. Greco R. S., Trooskin S. Z., Donetz A. P., Harvey R. A. The application of antibiotic bonding to the treatment of established vascular prosthetic infection. Arch Surg. 1985 Jan;120(1):71–75. doi: 10.1001/archsurg.1985.01390250063010. [DOI] [PubMed] [Google Scholar]
  15. Greisler H. P., Kim D. U., Price J. B., Voorhees A. B., Jr Arterial regenerative activity after prosthetic implantation. Arch Surg. 1985 Mar;120(3):315–323. doi: 10.1001/archsurg.1985.01390270055010. [DOI] [PubMed] [Google Scholar]
  16. Habal M. B. The biologic basis for the clinical application of the silicones. A correlate to their biocompatibility. Arch Surg. 1984 Jul;119(7):843–848. doi: 10.1001/archsurg.1984.01390190081019. [DOI] [PubMed] [Google Scholar]
  17. Kelly J. A., Dideberg O., Charlier P., Wery J. P., Libert M., Moews P. C., Knox J. R., Duez C., Fraipont C., Joris B. On the origin of bacterial resistance to penicillin: comparison of a beta-lactamase and a penicillin target. Science. 1986 Mar 21;231(4744):1429–1431. doi: 10.1126/science.3082007. [DOI] [PubMed] [Google Scholar]
  18. Kwaan J. H., Dahl R. K., Connolly J. Immunocompetence in patients with prosthetic graft infection. J Vasc Surg. 1984 Jan;1(1):45–49. [PubMed] [Google Scholar]
  19. Lowy F. D., Hammer S. M. Staphylococcus epidermidis infections. Ann Intern Med. 1983 Dec;99(6):834–839. doi: 10.7326/0003-4819-99-6-834. [DOI] [PubMed] [Google Scholar]
  20. MIRKOVITCH V., BECK R. E., ANDRUS P. G., LEININGER R. I. THE ZETA POTENTIALS AND BLOOD COMPATIBILITY CHARACTERISTICS OF SOME SELECTED SOLIDS. J Surg Res. 1964 Sep;4:395–399. doi: 10.1016/s0022-4804(64)80111-6. [DOI] [PubMed] [Google Scholar]
  21. Macbeth G. A., Rubin J. R., McIntyre K. E., Jr, Goldstone J., Malone J. M. The relevance of arterial wall microbiology to the treatment of prosthetic graft infections: graft infection vs. arterial infection. J Vasc Surg. 1984 Nov;1(6):750–756. doi: 10.1067/mva.1984.avs0010750. [DOI] [PubMed] [Google Scholar]
  22. Merritt K., Shafer J. W., Brown S. A. Implant site infection rates with porous and dense materials. J Biomed Mater Res. 1979 Jan;13(1):101–108. doi: 10.1002/jbm.820130111. [DOI] [PubMed] [Google Scholar]
  23. Papaevangelou G., Roumeliotou-Karayannis A., Contoyannis P. Changing epidemiological characteristics of acute viral hepatitis in Greece. Infection. 1982 Jan;10(1):1–4. doi: 10.1007/BF01640826. [DOI] [PubMed] [Google Scholar]
  24. Rosenman J. E., Kempczinski R. F., Berlatzky Y., Pearce W. H., Ramalanjaona G. R., Bjornson H. S. Bacterial adherence to endothelial-seeded polytetrafluoroethylene grafts. Surgery. 1985 Oct;98(4):816–823. [PubMed] [Google Scholar]
  25. Rosenman J. E., Pearce W. H., Kempczinski R. F. Bacterial adherence to vascular grafts after in vitro bacteremia. J Surg Res. 1985 Jun;38(6):648–655. doi: 10.1016/0022-4804(85)90088-5. [DOI] [PubMed] [Google Scholar]
  26. Schmitt D. D., Bandyk D. F., Pequet A. J., Malangoni M. A., Towne J. B. Mucin production by Staphylococcus epidermidis. A virulence factor promoting adherence to vascular grafts. Arch Surg. 1986 Jan;121(1):89–95. doi: 10.1001/archsurg.1986.01400010103013. [DOI] [PubMed] [Google Scholar]
  27. Schmitt D. D., Bandyk D. F., Pequet A. J., Towne J. B. Bacterial adherence to vascular prostheses. A determinant of graft infectivity. J Vasc Surg. 1986 May;3(5):732–740. [PubMed] [Google Scholar]
  28. Zimmerli W., Lew P. D., Waldvogel F. A. Pathogenesis of foreign body infection. Evidence for a local granulocyte defect. J Clin Invest. 1984 Apr;73(4):1191–1200. doi: 10.1172/JCI111305. [DOI] [PMC free article] [PubMed] [Google Scholar]

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