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. 1988 Feb;207(2):208–212. doi: 10.1097/00000658-198802000-00016

Lymphoid subpopulation changes after thermal injury and thermal injury with infection in an experimental model.

D G Burleson 1, A D Mason Jr 1, B A Pruitt Jr 1
PMCID: PMC1493365  PMID: 2963598

Abstract

Subpopulation analysis of peripheral blood lymphocytes is a frequently used measure of immunocompetence. Yet, little is known about the lymphocyte subpopulations in the circulation and lymphoid organs after severe trauma. Blood, spleen, and lymph node (LN) subpopulations were compared in a rat model of burn injury (B) and burn injury with infection (BI). B and BI rats received 30% total body surface scald burns. Infection was induced by seeding wounds with Pseudomonas aeruginosa. Subpopulations were identified by flow cytometry 48 hours after burn. Helper lymphocytes were selectively depleted from the circulation of BI but not B animals, which caused the ratio of helper to suppressor cells (HSR) in BI animals to decrease significantly compared with the unburned controls. Both LN helper and suppressor cells were decreased in BI animals and the HSR was unchanged, but a selective reduction in suppressor cells in B LN increased the HSR relative to unburned controls. Spleen subpopulations were unchanged for both B and BI groups. Subpopulation changes after trauma and infection were different for each tissue examined.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alexander J. W., Ogle C. K., Stinnett J. D., Macmillan B. G. A sequential, prospective analysis of immunologic abnormalities and infection following severe thermal injury. Ann Surg. 1978 Dec;188(6):809–816. doi: 10.1097/00000658-197812000-00016. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Antonacci A. C., Reaves L. E., Calvano S. E., Amand R., De Riesthal H. F., Shires G. T. Flow cytometric analysis of lymphocyte subpopulations after thermal injury in human beings. Surg Gynecol Obstet. 1984 Jul;159(1):1–8. [PubMed] [Google Scholar]
  3. Bjornson A. B., Altemeier W. A., Bjornson H. S. Host defense against opportunist microorganisms following trauma. II. Changes in complement and immunoglobulins in patients with abdominal trauma and in septic patients without trauma. Ann Surg. 1978 Jul;188(1):102–108. doi: 10.1097/00000658-197807000-00017. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Burleson D. G., Vaughn G. K., Mason A. D., Jr, Pruitt B. A., Jr Flow cytometric measurement of rat lymphocyte subpopulations after burn injury and burn injury with infection. Arch Surg. 1987 Feb;122(2):216–220. doi: 10.1001/archsurg.1987.01400140098013. [DOI] [PubMed] [Google Scholar]
  5. Kulick M. I., Lopez R. R. Immunological aspects of thermal injuries. Ann Plast Surg. 1981 Sep;7(3):252–255. [PubMed] [Google Scholar]
  6. McIrvine A. J., O'Mahony J. B., Saporoschetz I., Mannick J. A. Depressed immune response in burn patients: use of monoclonal antibodies and functional assays to define the role of suppressor cells. Ann Surg. 1982 Sep;196(3):297–304. doi: 10.1097/00000658-198209000-00008. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Meakins J. L. Clinical importance of host resistance to infection in surgical patients. Adv Surg. 1981;15:225–255. [PubMed] [Google Scholar]
  8. Munster A. M. Immunologic response of trauma and burns. An overview. Am J Med. 1984 Mar 30;76(3A):142–145. doi: 10.1016/0002-9343(84)90333-4. [DOI] [PubMed] [Google Scholar]
  9. Till G. O. Cellular and humoral defense systems and inflammatory mechanisms in thermal injury. Clin Lab Med. 1983 Dec;3(4):801–815. [PubMed] [Google Scholar]
  10. WALKER H. L., MASON A. D., Jr, RAULSTON G. L. SURFACE INFECTION WITH PSEUDOMONAS AERUGINOSA. Ann Surg. 1964 Aug;160:297–305. doi: 10.1097/00000658-196408000-00019. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Walker H. L., Mason A. D., Jr A standard animal burn. J Trauma. 1968 Nov;8(6):1049–1051. doi: 10.1097/00005373-196811000-00006. [DOI] [PubMed] [Google Scholar]
  12. Zapata-Sirvent R. L., Hansbrough J. F., Bender E. M., Bartle E. J., Mansour M. A., Carter W. H. Postburn immunosuppression in an animal model. IV. Improved resistance to septic challenge with immunomodulating drugs. Surgery. 1986 Jan;99(1):53–59. [PubMed] [Google Scholar]
  13. Zapata-Sirvent R. L., Hansbrough J. F. Postburn immunosuppression in an animal model. III. Maintenance of normal splenic helper and suppressor lymphocyte subpopulations by immunomodulating drugs. Surgery. 1985 Jun;97(6):721–727. [PubMed] [Google Scholar]

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