Abstract
Observations were made on the value of certain selective media for isolation and identification of staphylococci from milk obtained from individual quarters and farm bulk tanks Aerobic fermentation of mannitol salt was unreliable for identification of coagulase positive strains. Coagulase positive strains tended to clump when grown in tryptose serum broth. Tellurite glycine agar plates were not entirely reliable for the selection of coagulase positive strains. Fibrinogen-tellurite-glycine plates were very useful for the isolation of staphylococci from milk in the presence of other microorganisms and for the simultaneous identification of coagulase positive strains. Plate coagulase tests utilizing fibrinogen tellurite glycine agar corresponded almost perfectly with conventional tube tests. Fibrinolytic strains were not uncommon and were found also among beta hemolysin producing coagulase positive strains.
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Selected References
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- BROWN A. E., FARUQUE A. A. THE CLUMPING OF STAPHYLOCOCCUS PYOGENES BY SERUM AND FIBRINOGEN-FREE PLASMAS. J Clin Pathol. 1963 Sep;16:457–458. doi: 10.1136/jcp.16.5.457. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DENEKE A., BLOBEL H. Fibrinogen media for studies on staphylococci. J Bacteriol. 1962 Mar;83:533–537. doi: 10.1128/jb.83.3.533-537.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Joshi N., Dale D. G. Some Microbiological Aspects of Staphylococcal Mastitis. Can J Comp Med Vet Sci. 1963 Mar;27(3):61–68. [PMC free article] [PubMed] [Google Scholar]
- KIMLER A. Some clinical laboratory briefs on staphylococci. J Bacteriol. 1962 Jan;83:207–208. doi: 10.1128/jb.83.1.207-208.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LACK C. H. Plate coagulase and fibrinolysin tests for staphylococci. J Clin Pathol. 1957 Aug;10(3):208–210. doi: 10.1136/jcp.10.3.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LACK C. H., WAILLING D. G. A study of 435 strains of Staphylococcus pyogenes with reference to factors which may contribute to pathogenicity. J Pathol Bacteriol. 1954 Oct;68(2):431–443. doi: 10.1002/path.1700680217. [DOI] [PubMed] [Google Scholar]
- LOKEN K. I., HOYT H. H. Studies on bovine staphylococcic mastitis. I. Characterization of staphylococci. Am J Vet Res. 1962 May;23:534–540. [PubMed] [Google Scholar]
- REID W. B., WILSON J. B. A study of the staphylococci associated with the bovine udder. Am J Vet Res. 1959 Sep;20:825–831. [PubMed] [Google Scholar]
- RICHARDSON G. M. Chelating agents for selective growth of bacteria. Nature. 1961 Jan 7;189:78–79. doi: 10.1038/189078b0. [DOI] [PubMed] [Google Scholar]
- SLANETZ L. W., BARTLEY C. H. The diagnosis of staphylococcal mastitis, with special reference to the characteristics of mastitis staphylococci. J Infect Dis. 1953 Mar-Apr;92(2):139–151. doi: 10.1093/infdis/92.2.139. [DOI] [PubMed] [Google Scholar]
- YOTIS W. W., EKSTEDT R. D. Studies on staphylococci. I. Effect of serum and coagulase on the metabolism of coagulase positive and coagulase negative strains. J Bacteriol. 1959 Oct;78:567–574. doi: 10.1002/path.1700780225. [DOI] [PMC free article] [PubMed] [Google Scholar]