Abstract
The complement-fixation test was investigated as a means of detecting hog cholera virus in spleen from experimentally infected swine. Various methods of extracting the tissue for production of antigen are described and emphasis is placed on the necessity of using the modified direct complement-fixation test to obtain reactions. The tissue should be obtained from animals showing advanced clinical manifestations of the disease. Preferably, the tissue should be maintained frozen or at least well refrigerated. The results indicate that tissue from dead animals or from breeding sows should be avoided. The 77 per cent positive reactions obtained suggest the test could be of diagnostic value provided two or three samples are obtained from the same herd.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BANKOWSKI R. A., WICHMANN R., KUMMER M. A complement-fixation test for identification and differentiation of immunological types of the virus of vesicular exanthema of swine. Am J Vet Res. 1953 Apr;14(51):145–149. [PubMed] [Google Scholar]
- Boulanger P. Application Of The Complement-Fixation Test To The Demonstration Of Rinderpest Virus In The Tissue Of Infected Cattle Using Rabbit Antiserum. I. Results With The Kabete And Pendik Strains Of Virus. Can J Comp Med Vet Sci. 1957 Nov;21(11):379–388. [PMC free article] [PubMed] [Google Scholar]
- Boulanger P., Bannister G. L. Comparison of the Indirect and "Implemented" Direct Complement-Fixation Test in the Diagnosis of Turkey Ornithosis. Can J Comp Med Vet Sci. 1961 Jan;25(1):8–12. [PMC free article] [PubMed] [Google Scholar]
- Boulanger P. Complement-Fixation Tests Of Swine Serum. I. In The Diagnosis Of Vesicular Stomatitis. Can J Comp Med Vet Sci. 1955 Feb;19(2):37–47. [PMC free article] [PubMed] [Google Scholar]
- Boulanger P., Robertson A. Fluorescein-Labelled Antibody Technique for the Demonstration of Leptospira Pomona. Can J Comp Med Vet Sci. 1961 Dec;25(12):299–306. [PMC free article] [PubMed] [Google Scholar]
- Boulanger P. Technique of a Modified Direct Complement-Fixation Test for Viral Antibodies in Heat Inactivated Cattle Serum. Can J Comp Med Vet Sci. 1960 Sep;24(9):262–269. [PMC free article] [PubMed] [Google Scholar]
- COWAN K. M. Immunologic studies on African swine fever virus. II. Enhancing effect of normal bovine serum on the complement-fixation reaction. Am J Vet Res. 1963 Jul;24:756–761. [PubMed] [Google Scholar]
- Gutekunst D. E., Malmquist W. A. Complement-Fixing And Neutralizing Antibody Response To Bovine Viral Diarrhea And Hog Cholera Antigens. Can J Comp Med Vet Sci. 1964 Jan;28(1):19–23. [PMC free article] [PubMed] [Google Scholar]
- Robertson A., Bannister G. L., Ruckerbauer G. M., Boulanger P. Toxoplasmosis II. Studies of Animal Sera Using Complement-Fixation Tests. Can J Comp Med Vet Sci. 1963 Jul;27(7):169–176. [PMC free article] [PubMed] [Google Scholar]