Figure 3.
Direct association of SNAP23 on vimentin filaments. Human fibroblast were homogenized in a cell cracker, treated with cytochalasin B and nocodazole, and deposited on electron microscopy grids. The sample was double-stained for SNAP23 (10-nm gold particle, arrows) and vimentin (5-nm gold particle). The arrowhead points to a vimentin-bound vesicle, which is SNAP23-negative.